caa a22 4500 445292199 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s11626-010-9284-4 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9284-4 Derivation, culture, and characterization of VUB hESC lines [Elektronische Daten] [Ileana Mateizel, Claudia Spits, Martine De Rycke, Inge Liebaers, Karen Sermon] In this report, we present the derivation and characterization of 15hESC lines established at the Vrije Universiteit Brussel, Belgium in collaboration with the Universitair Ziekenhuis Brussel, Belgium, using surplus in vitro fertilization embryos and embryos carrying monogenic disorders donated for research. Four lines were derived from blastocyst-stage embryos presumed to be genetically normal, and 11hESC lines were obtained from embryos shown to carry genetic mutations by preimplantation genetic diagnosis. All the lines express markers of pluripotency as determined by immunocytochemistry and RT-PCR, and formed teratomas when injected into SCID mice. All VUB hESC lines, except for VUB17, are reported in the European hESC registry and are available upon request after signing a Material Transfer Agreement from the VUB (contact person: Prof. Dr. Karen Sermon; Karen.Sermon@uzbrussel.be). The Society for In Vitro Biology, 2010 hESC nationallicence Preimplantation genetic diagnosis nationallicence Mateizel Ileana Department of Embryology and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090, Brussels, Belgium aut Spits Claudia Department of Embryology and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090, Brussels, Belgium aut De Rycke Martine Department of Embryology and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090, Brussels, Belgium aut Liebaers Inge Department of Embryology and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090, Brussels, Belgium aut Sermon Karen Department of Embryology and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090, Brussels, Belgium aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 300-308 1071-2690 46:3-4<300 2010 46 11626 https://doi.org/10.1007/s11626-010-9284-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9284-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mateizel Ileana Department of Embryology and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090, Brussels, Belgium aut NATIONALLICENCE 700 1- Spits Claudia Department of Embryology and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090, Brussels, Belgium aut NATIONALLICENCE 700 1- De Rycke Martine Department of Embryology and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090, Brussels, Belgium aut NATIONALLICENCE 700 1- Liebaers Inge Department of Embryology and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090, Brussels, Belgium aut NATIONALLICENCE 700 1- Sermon Karen Department of Embryology and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090, Brussels, Belgium aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 300-308 1071-2690 46:3-4<300 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer