caa a22 4500 445292229 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s11626-010-9304-4 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9304-4 Efficient derivation of Chinese human embryonic stem cell lines from frozen embryos [Elektronische Daten] [Chunliang Li, Ying Yang, Xiaowei Lu, Yijuan Sun, Junjie Gu, Yun Feng, Ying Jin] Human embryonic stem (hES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Their unique properties of self-renewal and pluripotency make them an attractive tool for basic research as well as a potential cell resource for therapy. However, each hES cell line demonstrates different identity. It is desirable to obtain more fully characterized hES cell lines with newly developed technologies associated with hES cell culture. Here, we report our experience of efficient derivation of three new Chinese hES cell lines (SHhES2, SHhES3, and SHhES4) from in vitro fertilization discarded embryos donated by women with polycystic ovary syndrome. These cell lines were derived under conditions minimizing exposure to animal components and maintained at an undifferentiated state for long-term culture. They retained a normal karyotype and expressed ALP, OCT4, SOX2, SSEA-4, TRA-1-60 and TRA-1-81. RT-PCR analysis also revealed high expression levels of pluripotency markers such as OCT4, LEFTY A, SOX2, TDGF-1, THY1, FGF4, NANOG, and REX1. When suspended in low-attachment culture dishes, embryoid bodies formed and were comprised of various differentiated cell types from all three embryonic germ layers. However, well-shaped teratomas were only harvested from line SHhES2, not from SHhES3 and SHhES4, indicating that the differentiation ability in vivo differs among the three cell lines. Collectively, the three new hES cell lines were established and fully characterized. The effort paves the way toward generating hES cell lines without contamination by animal components. All of these cell lines are available by contact Ying Jin at yjin@sibs.ac.cn. The Society for In Vitro Biology, 2010 Human embryonic stem cells nationallicence Derivation nationallicence Characterization nationallicence Li Chunliang Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences/Shanghai Jiao Tong University School of Medicine, 225 South Chongqing Road, 200025, Shanghai, China aut Yang Ying Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences/Shanghai Jiao Tong University School of Medicine, 225 South Chongqing Road, 200025, Shanghai, China aut Lu Xiaowei Reproductive Medical Center, Ruijin Hospital, 197 Second Ruijin Road, 200135, Shanghai, China aut Sun Yijuan Reproductive Medical Center, Ruijin Hospital, 197 Second Ruijin Road, 200135, Shanghai, China aut Gu Junjie Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences/Shanghai Jiao Tong University School of Medicine, 225 South Chongqing Road, 200025, Shanghai, China aut Feng Yun Reproductive Medical Center, Ruijin Hospital, 197 Second Ruijin Road, 200135, Shanghai, China aut Jin Ying Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences/Shanghai Jiao Tong University School of Medicine, 225 South Chongqing Road, 200025, Shanghai, China aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 186-191 1071-2690 46:3-4<186 2010 46 11626 https://doi.org/10.1007/s11626-010-9304-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9304-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Chunliang Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences/Shanghai Jiao Tong University School of Medicine, 225 South Chongqing Road, 200025, Shanghai, China aut NATIONALLICENCE 700 1- Yang Ying Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences/Shanghai Jiao Tong University School of Medicine, 225 South Chongqing Road, 200025, Shanghai, China aut NATIONALLICENCE 700 1- Lu Xiaowei Reproductive Medical Center, Ruijin Hospital, 197 Second Ruijin Road, 200135, Shanghai, China aut NATIONALLICENCE 700 1- Sun Yijuan Reproductive Medical Center, Ruijin Hospital, 197 Second Ruijin Road, 200135, Shanghai, China aut NATIONALLICENCE 700 1- Gu Junjie Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences/Shanghai Jiao Tong University School of Medicine, 225 South Chongqing Road, 200025, Shanghai, China aut NATIONALLICENCE 700 1- Feng Yun Reproductive Medical Center, Ruijin Hospital, 197 Second Ruijin Road, 200135, Shanghai, China aut NATIONALLICENCE 700 1- Jin Ying Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences/Shanghai Jiao Tong University School of Medicine, 225 South Chongqing Road, 200025, Shanghai, China aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 186-191 1071-2690 46:3-4<186 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer