caa a22 4500 445292237 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100101xx s 000 0 eng 10.1007/s11626-009-9237-y doi (NATIONALLICENCE)springer-10.1007/s11626-009-9237-y Ailanthus altissima swingle has anti-anaphylactic effect and inhibits inflammatory cytokine expression via suppression of nuclear factor-kappaB activation [Elektronische Daten] [Tae-Hee Kang, In-Young Choi, Su-Jin Kim, Phil-Dong Moon, Jae-Uk Seo, Jae-Joong Kim, Nyeon-Hyung An, Sung-Hoon Kim, Min-Ho Kim, Jae-Young Um, Seung-Heon Hong, Hyung-Min Kim, Hyun-Ja Jeong] Ailanthus altissima swingle (ailanthic cortex, AAS) has been used as a traditional medicine for fever, bleeding, infection, and inflammation for many years in Korea. However, its mechanisms have not been examined. In the present study, we investigate the effect of AAS on the mast-cell-mediated allergic and inflammatory reaction using in vivo and in vitro models and elucidate its molecular mechanisms. AAS significantly inhibited compound 48/48-induced edema and systemic anaphylaxis. AAS significantly inhibited passive cutaneous anaphylaxis. AAS inhibited histamine release from rat peritoneal mast cells (RPMCs) in a dose-dependent manner. Moreover, AAS significantly inhibited production of inflammatory cytokines, tumor necrosis factor (TNF), interleukin (IL)-6, and IL-8 on the phorbol 12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-stimulated human mast cell line, HMC-1 cells. AAS inhibits the IgE or stem cell factor-induced TNF production on RPMCs. In activated HMC-1 cells, the expression level of NF-kappaB/Rel A protein increased in the nucleus, whereas the level of NF-kappaB/Rel A in the nucleus was decreased by AAS treatment. In addition, AAS inhibited the PMACI-induced IkappaBalpha degradation. In conclusion, the present results indicate that AAS has potent anti-anaphylactic and anti-inflammatory properties. The Society for In Vitro Biology, 2009 Ailanthus altissima swingle nationallicence Allergy·Inflammation·Cytokine·NF-kappaB nationallicence Kang Tae-Hee Cancer Preventive Material Development Research Center, Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, Kyung Hee, South Korea aut Choi In-Young Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, 130-701, Seoul, South Korea aut Kim Su-Jin Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, 130-701, Seoul, South Korea aut Moon Phil-Dong Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, 130-701, Seoul, South Korea aut Seo Jae-Uk Cancer Preventive Material Development Research Center, Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, Kyung Hee, South Korea aut Kim Jae-Joong Cancer Preventive Material Development Research Center, Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, Kyung Hee, South Korea aut An Nyeon-Hyung College of Pharmacy, VestibuloCochlear Research Center, Wonkwang University, 570-749, Iksan, Jeonbuk, South Korea aut Kim Sung-Hoon Cancer Preventive Material Development Research Center, Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, Kyung Hee, South Korea aut Kim Min-Ho NUMATE, Chonbuk National University, 561-756, Jeonbuk, Jeonju, South Korea aut Um Jae-Young Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, 130-701, Seoul, South Korea aut Hong Seung-Heon College of Pharmacy, VestibuloCochlear Research Center, Wonkwang University, 570-749, Iksan, Jeonbuk, South Korea aut Kim Hyung-Min Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, 130-701, Seoul, South Korea aut Jeong Hyun-Ja Biochip Research Center, Hoseo University, 165, Sechul-ri, Baebang-myun, Asan, Chungnam, 336-795 aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/1(2010-01-01), 72-81 1071-2690 46:1<72 2010 46 11626 https://doi.org/10.1007/s11626-009-9237-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9237-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kang Tae-Hee Cancer Preventive Material Development Research Center, Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, Kyung Hee, South Korea aut NATIONALLICENCE 700 1- Choi In-Young Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, 130-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Kim Su-Jin Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, 130-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Moon Phil-Dong Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, 130-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Seo Jae-Uk Cancer Preventive Material Development Research Center, Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, Kyung Hee, South Korea aut NATIONALLICENCE 700 1- Kim Jae-Joong Cancer Preventive Material Development Research Center, Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, Kyung Hee, South Korea aut NATIONALLICENCE 700 1- An Nyeon-Hyung College of Pharmacy, VestibuloCochlear Research Center, Wonkwang University, 570-749, Iksan, Jeonbuk, South Korea aut NATIONALLICENCE 700 1- Kim Sung-Hoon Cancer Preventive Material Development Research Center, Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, Kyung Hee, South Korea aut NATIONALLICENCE 700 1- Kim Min-Ho NUMATE, Chonbuk National University, 561-756, Jeonbuk, Jeonju, South Korea aut NATIONALLICENCE 700 1- Um Jae-Young Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, 130-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Hong Seung-Heon College of Pharmacy, VestibuloCochlear Research Center, Wonkwang University, 570-749, Iksan, Jeonbuk, South Korea aut NATIONALLICENCE 700 1- Kim Hyung-Min Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, 130-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Jeong Hyun-Ja Biochip Research Center, Hoseo University, 165, Sechul-ri, Baebang-myun, Asan, Chungnam, 336-795 aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/1(2010-01-01), 72-81 1071-2690 46:1<72 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer