caa a22 4500 445292245 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100101xx s 000 0 eng 10.1007/s11626-009-9245-y doi (NATIONALLICENCE)springer-10.1007/s11626-009-9245-y Isolation, culture and identification of epidermal stem cells from newborn mouse skin [Elektronische Daten] [Somayeh Reiisi, Fariba Esmaeili, Abolfazl Shirazi] In healthy individuals, skin integrity is maintained by epidermal stem cells which self-renew and generate daughter cells that undergo terminal differentiation. Epidermal stem cells represent a promising source of stem cells, and their culture has great potential in scientific research and clinical application. However, no single method has been universally adopted for identifying and isolating epidermal stem cells. Here, we reported the isolation and characterization of putative epidermal stem cells from newborn mouse skin. The keratinocytes were separated enzymatically. Putative epidermal stem cells were selected by rapid adherence on a composite matrix made of type I collagen and fibronectin. Unattached cells were discarded after 10min, and the attached cells were cultured in a defined culture medium. The isolated cells showed the typical epidermal stem cell morphology. Immunofluorescence indicated that the cells were strongly stained for β1 integrin family of extracellular matrix receptors. In conclusion, mouse putative epidermal stem cells were successfully isolated from newborn mouse epidermis on the basis of high rapid adhesion to extracellular matrix proteins and cultured in vitro. The Society for In Vitro Biology, 2009 Epidermal stem cells nationallicence Mouse skin keratinocytes nationallicence β1 integrin nationallicence Stem cell isolation nationallicence Reiisi Somayeh Department of Biology, Faculty of Basic Sciences, University of Shahrekord, Shahrekord, Iran aut Esmaeili Fariba Department of Biology, Faculty of Basic Sciences, University of Shahrekord, Shahrekord, Iran aut Shirazi Abolfazl Research Institute of Animal Embryo Technology, University of Shahrekord, Shahrekord, Iran aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/1(2010-01-01), 54-59 1071-2690 46:1<54 2010 46 11626 https://doi.org/10.1007/s11626-009-9245-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9245-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reiisi Somayeh Department of Biology, Faculty of Basic Sciences, University of Shahrekord, Shahrekord, Iran aut NATIONALLICENCE 700 1- Esmaeili Fariba Department of Biology, Faculty of Basic Sciences, University of Shahrekord, Shahrekord, Iran aut NATIONALLICENCE 700 1- Shirazi Abolfazl Research Institute of Animal Embryo Technology, University of Shahrekord, Shahrekord, Iran aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/1(2010-01-01), 54-59 1071-2690 46:1<54 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer