caa a22 4500 445292253 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100101xx s 000 0 eng 10.1007/s11626-009-9243-0 doi (NATIONALLICENCE)springer-10.1007/s11626-009-9243-0 A new approach for determination of Na,K-ATPase activity: application to intact pancreatic β-cells [Elektronische Daten] [Ana Costa, Joana Real, Célia Antunes, Júlio Cruz-Morais] It has been postulated that a decrease in Na,K-ATPase-mediated ion gradients may be a contributing mechanism to insulin secretion. However, the precise role of the Na,K-ATPase in pancreatic β-cell membrane depolarization and insulin secretion signalling have been difficult to evaluate, mostly because data reporting changes in enzymatic activity have been obtained in cell homogenates or membrane preparations, lacking intact intracellular signalling pathways. The aim of this work was to develop a method to characterize Na,K-ATPase activity in intact pancreatic β-cells that will allow the investigation of putative Na,K-ATPase activity regulation by glucose and its possible role in insulin secretion signalling. This work demonstrates for the first time that it is possible to determine Na,K-ATPase activity in intact pancreatic β-cells and that this is a suitable method for the study of the mechanisms involved in the Na,K-ATPase regulation and eventually its relevance for insulin secretion signalling. The Society for In Vitro Biology, 2009 Pancreatic β-cell nationallicence Na,K-ATPase nationallicence Enzymatic activity nationallicence Insulin secretion nationallicence Costa Ana Department of Chemistry, University of Évora, Rua Romao Ramalho, 59, 7000 757, Évora, Portugal aut Real Joana Centre for Neuroscience and Cell Biology (CNC), Coimbra, Portugal aut Antunes Célia Department of Chemistry, University of Évora, Rua Romao Ramalho, 59, 7000 757, Évora, Portugal aut Cruz-Morais Júlio Department of Chemistry, University of Évora, Rua Romao Ramalho, 59, 7000 757, Évora, Portugal aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/1(2010-01-01), 7-10 1071-2690 46:1<7 2010 46 11626 https://doi.org/10.1007/s11626-009-9243-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9243-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Costa Ana Department of Chemistry, University of Évora, Rua Romao Ramalho, 59, 7000 757, Évora, Portugal aut NATIONALLICENCE 700 1- Real Joana Centre for Neuroscience and Cell Biology (CNC), Coimbra, Portugal aut NATIONALLICENCE 700 1- Antunes Célia Department of Chemistry, University of Évora, Rua Romao Ramalho, 59, 7000 757, Évora, Portugal aut NATIONALLICENCE 700 1- Cruz-Morais Júlio Department of Chemistry, University of Évora, Rua Romao Ramalho, 59, 7000 757, Évora, Portugal aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/1(2010-01-01), 7-10 1071-2690 46:1<7 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer