caa a22 4500 445292288 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20100101xx s 000 0 eng 10.1007/s11626-009-9239-9 doi (NATIONALLICENCE)springer-10.1007/s11626-009-9239-9 TGF-β3 is expressed in taste buds and inhibits proliferation of primary cultured taste epithelial cells [Elektronische Daten] [Shin-ichi Nakamura, Takayuki Kawai, Takashi Kamakura, Tetsuya Ookura] Transforming growth factor-βs (TGF-βs), expressed in various tissues, play important roles in embryonic development and adult tissue homeostasis through their effects on cell proliferation, cell differentiation, cell death, and cell motility. However, expression of TGF-β signaling components and their biological effect on taste epithelia has not been elucidated. We performed expression analysis of TGF-β signaling components in taste epithelia and found that the TGF-β3 mRNA was specifically expressed in taste buds. Type II TGF-βs receptor (TβR-II) mRNA was specifically expressed in the tongue epithelia including the taste epithelia. To elucidate the biological function of TGF-β3 in taste epithelia, we performed proliferation assay with primary cultured taste epithelial cells. In the presence of TGF-β3, percentage of BrdU-labeled cells decreased significantly, suggesting that the TGF-β3 inhibited the proliferation of cultured taste epithelial cells through inhibiting cell-cycle entry into S phase. By quantitative reverse transcription-polymerase chain reaction assay, we found that the TGF-β3 resulted in an increased level of expression of p15Ink4b and p21Cip1, suggesting that the TGF-β3 inhibited the taste epithelial cell proliferation through inhibiting G1cyclin-Cdk complexes. Taken together, these results suggested that the TGF-β3 may regulate taste epithelial cell homeostasis through controlling cell proliferation. The Society for In Vitro Biology, 2009 TGF-β3 nationallicence Taste buds nationallicence Proliferation nationallicence Primary cell culture nationallicence Taste epithelia nationallicence Nakamura Shin-ichi Food Function Division, National Food Research Institute, National Agriculture and Food Research Organization, Kannondai 2-1-12, 305-8642, Tsukuba, Ibaraki, Japan aut Kawai Takayuki Food Function Division, National Food Research Institute, National Agriculture and Food Research Organization, Kannondai 2-1-12, 305-8642, Tsukuba, Ibaraki, Japan aut Kamakura Takashi Department of Applied Biological Science, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut Ookura Tetsuya Food Function Division, National Food Research Institute, National Agriculture and Food Research Organization, Kannondai 2-1-12, 305-8642, Tsukuba, Ibaraki, Japan aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/1(2010-01-01), 36-44 1071-2690 46:1<36 2010 46 11626 https://doi.org/10.1007/s11626-009-9239-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9239-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakamura Shin-ichi Food Function Division, National Food Research Institute, National Agriculture and Food Research Organization, Kannondai 2-1-12, 305-8642, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Kawai Takayuki Food Function Division, National Food Research Institute, National Agriculture and Food Research Organization, Kannondai 2-1-12, 305-8642, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Kamakura Takashi Department of Applied Biological Science, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Ookura Tetsuya Food Function Division, National Food Research Institute, National Agriculture and Food Research Organization, Kannondai 2-1-12, 305-8642, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/1(2010-01-01), 36-44 1071-2690 46:1<36 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer