caa a22 4500 445292296 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20100101xx s 000 0 eng 10.1007/s11626-009-9236-z doi (NATIONALLICENCE)springer-10.1007/s11626-009-9236-z The proliferative effects of retinoic acid on primary cultures of adult rat type II pneumocytes depend upon cell density [Elektronische Daten] [Richard Baybutt, Brendon Smith, Elena Donskaya, Ling Hu, Ting Li, Weiqun Wang] Retinoic acid (RA) is important for maintaining integrity of alveolar epithelial cells, but the mechanism has not been defined. We cultured type II pneumocytes at confluent, high cell density (104 cells/mm2) and found that RA (10−6 M) inhibited thymidine incorporation to 60% of control, despite a dose-dependent increase in epidermal growth factor receptor (EGFR) levels. However, at lower, subconfluent density (102 cells/mm2), RA stimulated thymidine incorporation to 280% of control. EGF increased thymidine incorporation at concentrations as low as 0.1ng/mL, but no further increase was observed at higher concentrations up to 100ng/mL. In subconfluent cells co-treated with EGF (100ng/mL) and increasing concentrations of RA (10−8 M-10−5 M RA), thymidine incorporation was significantly greater at all concentrations than RA alone, with greatest increases observed at 10−7 (422% of control) and 10−6 (470% of control) M RA. In summary, the effects of RA on thymidine incorporation are sensitive to changes in cell density. RA inhibits thymidine incorporation at high cell density and stimulates thymidine incorporation at low density. RA increases EGFRs in cultured type II pneumocytes, and EGF stimulates thymidine incorporation independent of the cultured cell density. These data may help to explain how RA mediates lung repair in vivo. The Society for In Vitro Biology, 2009 All-trans retinoic acid nationallicence Epidermal growth factor nationallicence Thymidine nationallicence Pulmonary alveoli nationallicence B-EGF : Biotin-labeled EGF nationallicence BSA : Bovine serum albumin nationallicence DMEM : Dulbecco's modified Eagle's medium nationallicence EGF : Epidermal growth factor nationallicence EGFR : Epidermal growth factor receptor nationallicence ELISA : Enzyme-linked immunosorbent assay nationallicence FBS : Fetal bovine serum nationallicence FITC : Fluorescein isothiocyanate nationallicence Mab : Monoclonal antibody nationallicence PBS : Phosphate-buffered saline nationallicence RA : Retinoic acid nationallicence [3H]thymidine : Tritiated thymidine nationallicence Baybutt Richard Applied Health Science Department, Wheaton College, 501 College Ave, 60187, Wheaton, IL, USA aut Smith Brendon Applied Health Science Department, Wheaton College, 501 College Ave, 60187, Wheaton, IL, USA aut Donskaya Elena Department of Human Nutrition, College of Human Ecology, Kansas State University, 119 Justin Hall, 66506, Manhattan, KS, USA aut Hu Ling Department of Human Nutrition, College of Human Ecology, Kansas State University, 119 Justin Hall, 66506, Manhattan, KS, USA aut Li Ting Department of Human Nutrition, College of Human Ecology, Kansas State University, 119 Justin Hall, 66506, Manhattan, KS, USA aut Wang Weiqun Department of Human Nutrition, College of Human Ecology, Kansas State University, 119 Justin Hall, 66506, Manhattan, KS, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/1(2010-01-01), 20-27 1071-2690 46:1<20 2010 46 11626 https://doi.org/10.1007/s11626-009-9236-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9236-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baybutt Richard Applied Health Science Department, Wheaton College, 501 College Ave, 60187, Wheaton, IL, USA aut NATIONALLICENCE 700 1- Smith Brendon Applied Health Science Department, Wheaton College, 501 College Ave, 60187, Wheaton, IL, USA aut NATIONALLICENCE 700 1- Donskaya Elena Department of Human Nutrition, College of Human Ecology, Kansas State University, 119 Justin Hall, 66506, Manhattan, KS, USA aut NATIONALLICENCE 700 1- Hu Ling Department of Human Nutrition, College of Human Ecology, Kansas State University, 119 Justin Hall, 66506, Manhattan, KS, USA aut NATIONALLICENCE 700 1- Li Ting Department of Human Nutrition, College of Human Ecology, Kansas State University, 119 Justin Hall, 66506, Manhattan, KS, USA aut NATIONALLICENCE 700 1- Wang Weiqun Department of Human Nutrition, College of Human Ecology, Kansas State University, 119 Justin Hall, 66506, Manhattan, KS, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/1(2010-01-01), 20-27 1071-2690 46:1<20 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer