caa a22 4500 445292318 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20100101xx s 000 0 eng 10.1007/s11626-009-9240-3 doi (NATIONALLICENCE)springer-10.1007/s11626-009-9240-3 Differentiation of human adipose-derived stem cells induced by recombinantly expressed fibroblast growth factor 10 in vitro and in vivo [Elektronische Daten] [Xunyi Zhang, Minjuan Wu, Weiwei Zhang, Jiangfan Shen, Houqi Liu] The adipogenesis effect of fibroblast growth factor 10 (FGF10) has been demonstrated in many studies. The aim of this study is to render a novel method which can continuously induce hypodermal adipose-derived stem cell (ADSC) differentiation and maturation in vivo and in vitro using FGF10. We constructed a recombinant pcDNA3.0-FGF10-MSC which can continuously express FGF10 by transfected FGF10 into a human mesenchymal stem cell (MSC) clone, and we cultured ADSCs from human subcutaneous resected adipose tissue. An in vitro and in vivo co-culture system of pcDNA3.0-FGF10-MSC and ADSCs was then established. We observed the characteristics of ADSCs, monitored the adipogenesis-related transcription factor CAAT/enhancer binding protein-β, peroxisome proliferator-activated receptor-γ, and measured the adipose tissue layer of carrier animals. The results showed that FGF10 secreted from pcDNA3.0-FGF10-MSC could induce ADSC differentiation into mature adipocytes consistently. The study demonstrated that FGF10 can promote the adipogenesis effect in situ, and the autotransplantation of a carrier continuously secreting FGF10 may be utilized for increasing local subcutaneous adipose tissue in cosmetology. The Society for In Vitro Biology, 2009 FGF10 nationallicence ADSC nationallicence Transcription factor nationallicence Adipogenesis nationallicence Zhang Xunyi Nuclear Medicine Department, Shanghai University of Traditional Chinese Medicine affiliated PuTuo Hospital, PuTuo District, No. 164, LanXi Rd, 200062, Shanghai, China aut Wu Minjuan Research Center of Developmental Biology, Second Military Medical University, 200433, Shanghai, China aut Zhang Weiwei Laboratory Diagnosis Department, Second Military Medical University affiliated Changhai Hospital, 200433, Shanghai, China aut Shen Jiangfan Nuclear Medicine Department, Shanghai University of Traditional Chinese Medicine affiliated PuTuo Hospital, 200062, Shanghai, China aut Liu Houqi Research Center of Developmental Biology, Second Military Medical University, 200433, Shanghai, China aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/1(2010-01-01), 60-71 1071-2690 46:1<60 2010 46 11626 https://doi.org/10.1007/s11626-009-9240-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9240-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Xunyi Nuclear Medicine Department, Shanghai University of Traditional Chinese Medicine affiliated PuTuo Hospital, PuTuo District, No. 164, LanXi Rd, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Wu Minjuan Research Center of Developmental Biology, Second Military Medical University, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Zhang Weiwei Laboratory Diagnosis Department, Second Military Medical University affiliated Changhai Hospital, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Shen Jiangfan Nuclear Medicine Department, Shanghai University of Traditional Chinese Medicine affiliated PuTuo Hospital, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Liu Houqi Research Center of Developmental Biology, Second Military Medical University, 200433, Shanghai, China aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/1(2010-01-01), 60-71 1071-2690 46:1<60 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer