caa a22 4500 445292326 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20100101xx s 000 0 eng 10.1007/s11626-009-9241-2 doi (NATIONALLICENCE)springer-10.1007/s11626-009-9241-2 The Trichoplusia ni cell line MSU-TnT4 does not harbor a latent nodavirus [Elektronische Daten] [Fengrui Zhang, Suzanne Thiem] MSU-TnT4 (TnT4) cells, a newly established Trichoplusia ni cell line, was examined for the presence of a latent nodavirus, as had been described for another T. ni cell line, BTI-TN-5B1-4 (Hi5) cells. Reverse transcriptase polymerase chain reaction using nodavirus-specific primers did not detect virus genomic RNA in TnT4 cells. Transmission electron microscopy of recombinant baculovirus-infected TnT4 cells showed no evidence of latent nodavirus activation. Nodavirus particles were not detected in density gradients of baculovirus-infected TnT4 cell lysates or cell supernatants. The same methods confirmed the presence of a latent nodavirus in Hi5 cells. The Society for In Vitro Biology, 2009 Baculovirus expression vector system nationallicence BEVS nationallicence Trichoplusia ni nationallicence Insect cell line nationallicence BTI-Tn-5B1-4 nationallicence Hi5 nationallicence Zhang Fengrui Department of Entomology, Michigan State University, 48824, East Lansing, MI, USA aut Thiem Suzanne Department of Entomology, Michigan State University, 48824, East Lansing, MI, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/1(2010-01-01), 1-6 1071-2690 46:1<1 2010 46 11626 https://doi.org/10.1007/s11626-009-9241-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9241-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Fengrui Department of Entomology, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Thiem Suzanne Department of Entomology, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/1(2010-01-01), 1-6 1071-2690 46:1<1 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer