caa a22 4500 445292342 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20100601xx s 000 0 eng 10.1007/s11626-010-9292-4 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9292-4 RETRACTED ARTICLE: Isolation and characterization of goat retinal microvascular endothelial cells [Elektronische Daten] [Ravinarayanan Haribalaganesh, Elayappan Banumathi, Sardarpasha Sheikpranbabu, Venkataraman Deepak, Namagiri Sirishkumar, Sangiliyandi Gurunathan] In this report, we demonstrate a method for the isolation of pure homogeneous endothelial cell population from goat's eye without using multi-step procedure and sophisticated instrument facilities. Microvascular endothelial cell from goat's retina were isolated using enzymatic method and cultured in Iscove's Modified Dulbecco's Medium supplemented with 10% fetal bovine serum. Five to 7d after plating, a monolayer of endothelial cells was formed. These cells were identified as endothelial cells by morphology and confirmed by positive immunocytochemistry for vWF, CD31, VE-cadherin, CD146, VCAM-1, and ICAM-1, a specific marker for endothelial cells. We have compared both the mechanical and non-mechanical enzymatic methods in isolating pure endothelial cells. Cells plated on 4% gelatin-coated dishes resulted in tubular morphology, a characteristic of endothelial cells. This method is simpler and cost-effective when compared with other previously reported methods. These endothelial cells will be more helpful to identify the role of various factors in angiogenic-related disease such as diabetic retinopathy. The Society for In Vitro Biology, 2010 Diabetic retinopathy nationallicence Retinal endothelial cells nationallicence Angiogenesis nationallicence Endothelial characterization nationallicence Haribalaganesh Ravinarayanan Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, 626 190, Krishnankoil, Tamilnadu, India aut Banumathi Elayappan Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, 626 190, Krishnankoil, Tamilnadu, India aut Sheikpranbabu Sardarpasha Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, 626 190, Krishnankoil, Tamilnadu, India aut Deepak Venkataraman Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, 626 190, Krishnankoil, Tamilnadu, India aut Sirishkumar Namagiri Department of Ophthalmology, Meenakshi Mission Hospital and Research Center, 625 107, Madurai, Tamilnadu, India aut Gurunathan Sangiliyandi Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, 626 190, Krishnankoil, Tamilnadu, India aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/6(2010-06-01), 529-537 1071-2690 46:6<529 2010 46 11626 https://doi.org/10.1007/s11626-010-9292-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9292-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Haribalaganesh Ravinarayanan Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, 626 190, Krishnankoil, Tamilnadu, India aut NATIONALLICENCE 700 1- Banumathi Elayappan Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, 626 190, Krishnankoil, Tamilnadu, India aut NATIONALLICENCE 700 1- Sheikpranbabu Sardarpasha Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, 626 190, Krishnankoil, Tamilnadu, India aut NATIONALLICENCE 700 1- Deepak Venkataraman Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, 626 190, Krishnankoil, Tamilnadu, India aut NATIONALLICENCE 700 1- Sirishkumar Namagiri Department of Ophthalmology, Meenakshi Mission Hospital and Research Center, 625 107, Madurai, Tamilnadu, India aut NATIONALLICENCE 700 1- Gurunathan Sangiliyandi Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, 626 190, Krishnankoil, Tamilnadu, India aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/6(2010-06-01), 529-537 1071-2690 46:6<529 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer