caa a22 4500 445292350 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20100601xx s 000 0 eng 10.1007/s11626-009-9271-9 doi (NATIONALLICENCE)springer-10.1007/s11626-009-9271-9 Parasiticidal activity of human α-defensin-5 against Toxoplasma gondii [Elektronische Daten] [Tetsuya Tanaka, Md. Rahman, Banzragch Battur, Damdinsuren Boldbaatar, Min Liao, Rika Umemiya-Shirafuji, Xuenan Xuan, Kozo Fujisaki] Human defensins play a fundamental role in the initiation of innate immune responses to some microbial pathogens. In this paper, we show that human α-defensin-5 displays a parasiticidal role against Toxoplasma gondii, the causative agent of toxoplasmosis. Exposure of the tachyzoite form of T. gondii to defensin induced aggregation and significantly reduced parasite viability in a concentration-dependent peptide. Pre-incubation of tachyzoites with human α-defensin-5 followed by exposure to a mouse embryonal cell line (NIH/3T3) significantly reduced T. gondii infection in these cells. Thus, human α-defensin-5 is an innate immune molecule that causes severe toxocity to T. gondii and plays an important role in reducing cellular infection. This is the first report showing that human α-defensin-5 causes aggregation, leading to Toxoplasma destruction. The Society for In Vitro Biology, 2010 Human α-defensin-5 nationallicence Toxoplasma gondii nationallicence Aggregation nationallicence Parasiticidal activity nationallicence Tanaka Tetsuya Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Science, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, 890-0065, Kagoshima, Japan aut Rahman Md Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Science, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, 890-0065, Kagoshima, Japan aut Battur Banzragch Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Science, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, 890-0065, Kagoshima, Japan aut Boldbaatar Damdinsuren Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Science, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, 890-0065, Kagoshima, Japan aut Liao Min Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Science, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, 890-0065, Kagoshima, Japan aut Umemiya-Shirafuji Rika Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Science, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, 890-0065, Kagoshima, Japan aut Xuan Xuenan National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, 080-8555, Obihiro, Hokkaido, Japan aut Fujisaki Kozo Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Science, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, 890-0065, Kagoshima, Japan aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/6(2010-06-01), 560-565 1071-2690 46:6<560 2010 46 11626 https://doi.org/10.1007/s11626-009-9271-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9271-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tanaka Tetsuya Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Science, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, 890-0065, Kagoshima, Japan aut NATIONALLICENCE 700 1- Rahman Md Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Science, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, 890-0065, Kagoshima, Japan aut NATIONALLICENCE 700 1- Battur Banzragch Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Science, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, 890-0065, Kagoshima, Japan aut NATIONALLICENCE 700 1- Boldbaatar Damdinsuren Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Science, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, 890-0065, Kagoshima, Japan aut NATIONALLICENCE 700 1- Liao Min Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Science, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, 890-0065, Kagoshima, Japan aut NATIONALLICENCE 700 1- Umemiya-Shirafuji Rika Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Science, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, 890-0065, Kagoshima, Japan aut NATIONALLICENCE 700 1- Xuan Xuenan National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, 080-8555, Obihiro, Hokkaido, Japan aut NATIONALLICENCE 700 1- Fujisaki Kozo Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Science, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, 890-0065, Kagoshima, Japan aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/6(2010-06-01), 560-565 1071-2690 46:6<560 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer