caa a22 4500 445292369 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20100601xx s 000 0 eng 10.1007/s11626-010-9309-z doi (NATIONALLICENCE)springer-10.1007/s11626-010-9309-z Matched pairs of human prostate stromal cells display differential tropic effects on LNCaP prostate cancer cells [Elektronische Daten] [Xiaojuan Sun, Hui He, Zhihui Xie, Weiping Qian, Haiyen Zhau, Leland Chung, Fray Marshall, Ruoxiang Wang] Prostate stromal cells may play binary roles in the process of prostate cancer development. As the first to be encountered by infiltrating prostate cancer cells, prostate stromal cells form the first defense line against prostate cancer progression and metastasis. However, interaction between prostate cancer and stromal cells may facilitate the formation of a tumor microenvironment favoring cancer cell growth and survival. To establish an experimental system for studying the interaction between cancer and stromal cells, we isolated three matched pairs of normal and cancer-associated human prostate stromal clones. In this report, we describe the morphologic and behavioral characteristics of these cells and their effect on LNCaP prostate cancer cells in co-culture. Unlike LNCaP prostate cancer cells, the isolated prostate stromal clones are large fibroblast-like cells with a slow proliferation rate. Growth and survival of these clones are not affected by androgens. The stromal cells display high resistance to serum starvation, while cancer-associated stromal clones have differentiated survival ability. In co-culture experiments, the stromal cells protected some LNCaP prostate cancer cells from death by serum starvation, and cancer-associated stromal clones showed more protection. This work thus established a panel of valuable human prostate stromal cell lines, which could be used in co-culture to study the interaction between prostate cancer and prostate stromal cells. The Author(s), 2010 Prostate stromal cells nationallicence Prostate cancer nationallicence Cancer-stromal interaction nationallicence Tumor microenvironment nationallicence Cell survival nationallicence Sun Xiaojuan Molecular Urology and Therapeutics, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, 1365B Clifton Road, NE, Suite B5103, 30322, Atlanta, GA, USA aut He Hui Molecular Urology and Therapeutics, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, 1365B Clifton Road, NE, Suite B5103, 30322, Atlanta, GA, USA aut Xie Zhihui Molecular Urology and Therapeutics, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, 1365B Clifton Road, NE, Suite B5103, 30322, Atlanta, GA, USA aut Qian Weiping Molecular Urology and Therapeutics, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, 1365B Clifton Road, NE, Suite B5103, 30322, Atlanta, GA, USA aut Zhau Haiyen Molecular Urology and Therapeutics, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, 1365B Clifton Road, NE, Suite B5103, 30322, Atlanta, GA, USA aut Chung Leland Molecular Urology and Therapeutics, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, 1365B Clifton Road, NE, Suite B5103, 30322, Atlanta, GA, USA aut Marshall Fray Molecular Urology and Therapeutics, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, 1365B Clifton Road, NE, Suite B5103, 30322, Atlanta, GA, USA aut Wang Ruoxiang Molecular Urology and Therapeutics, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, 1365B Clifton Road, NE, Suite B5103, 30322, Atlanta, GA, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/6(2010-06-01), 538-546 1071-2690 46:6<538 2010 46 11626 https://doi.org/10.1007/s11626-010-9309-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9309-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun Xiaojuan Molecular Urology and Therapeutics, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, 1365B Clifton Road, NE, Suite B5103, 30322, Atlanta, GA, USA aut NATIONALLICENCE 700 1- He Hui Molecular Urology and Therapeutics, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, 1365B Clifton Road, NE, Suite B5103, 30322, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Xie Zhihui Molecular Urology and Therapeutics, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, 1365B Clifton Road, NE, Suite B5103, 30322, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Qian Weiping Molecular Urology and Therapeutics, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, 1365B Clifton Road, NE, Suite B5103, 30322, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Zhau Haiyen Molecular Urology and Therapeutics, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, 1365B Clifton Road, NE, Suite B5103, 30322, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Chung Leland Molecular Urology and Therapeutics, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, 1365B Clifton Road, NE, Suite B5103, 30322, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Marshall Fray Molecular Urology and Therapeutics, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, 1365B Clifton Road, NE, Suite B5103, 30322, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Wang Ruoxiang Molecular Urology and Therapeutics, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, 1365B Clifton Road, NE, Suite B5103, 30322, Atlanta, GA, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/6(2010-06-01), 538-546 1071-2690 46:6<538 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer