caa a22 4500 445292377 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20100601xx s 000 0 eng 10.1007/s11626-010-9296-0 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9296-0 A critical role of redox state in determining HL-60 cell granulocytic differentiation and apoptosis via involvement of PKC and NF-κB [Elektronische Daten] [Jurate Savickiene, Grazina Treigyte, Arunas Gineitis, Ruta Navakauskiene] The modifications of intracellular redox balance leads to important cellular changes in many cell types. Here, a causal relationship among redox state, granulocytic differentiation induced by all-trans retinoic acid (RA) or dibutyryl cAMP (dbcAMP) and apoptosis have been studied in the human acute promyelocytic leukaemia HL-60 cells. The modulation of intracellular reactive oxygen species levels by d, l-buthionine-(S, R) sulfoximide (BSO), and n-acetyl-l-cysteine (NAC) caused inducer- and time-dependent or stage-specific effects on HL-60 cell growth inhibition, differentiation and subsequent apoptosis. The presence of BSO during the commitment stage suppressed RA—but not dbcAMP-mediated differentiation, while NAC inhibited both. BSO alone and in combination with RA or dbcAMP-induced apoptosis, which was prevented by NAC in dbcAMP—but not in RA-treated cells. Using protein kinase C inhibitor, calphostin C, cross-talk effects between the intracellular redox state and PKC signalling was identified by demonstrating inducer-dependent changes in cell differentiation or apoptosis, which were associated with the changes in DNA-NF-κB binding activity. These observations suggest a critical role of redox state in determining HL-60 cell behaviour and provide new insights into the complex effects of redox perturbations on the intracellular signalling network via the involvement of PKC and NF-κB. The Society for In Vitro Biology, 2010 Granulocytic differentiation nationallicence Apoptosis nationallicence ROS nationallicence PKC nationallicence NF-κB nationallicence Savickiene Jurate Department of Developmental Biology, Institute of Biochemistry, Mokslininku st. 12, 08662, Vilnius, Lithuania aut Treigyte Grazina Department of Developmental Biology, Institute of Biochemistry, Mokslininku st. 12, 08662, Vilnius, Lithuania aut Gineitis Arunas Department of Developmental Biology, Institute of Biochemistry, Mokslininku st. 12, 08662, Vilnius, Lithuania aut Navakauskiene Ruta Department of Developmental Biology, Institute of Biochemistry, Mokslininku st. 12, 08662, Vilnius, Lithuania aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/6(2010-06-01), 547-559 1071-2690 46:6<547 2010 46 11626 https://doi.org/10.1007/s11626-010-9296-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9296-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Savickiene Jurate Department of Developmental Biology, Institute of Biochemistry, Mokslininku st. 12, 08662, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Treigyte Grazina Department of Developmental Biology, Institute of Biochemistry, Mokslininku st. 12, 08662, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Gineitis Arunas Department of Developmental Biology, Institute of Biochemistry, Mokslininku st. 12, 08662, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Navakauskiene Ruta Department of Developmental Biology, Institute of Biochemistry, Mokslininku st. 12, 08662, Vilnius, Lithuania aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/6(2010-06-01), 547-559 1071-2690 46:6<547 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer