caa a22 4500 445292385 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20100601xx s 000 0 eng 10.1007/s11626-010-9272-8 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9272-8 Epithelial-mesenchymal transition in rhesus monkey embryonic stem cell colonies: the role of culturing conditions [Elektronische Daten] [Birgit Maranca-Hüwel, Hans-Werner Denker] Colonies of rhesus monkey embryonic stem cells (rhESC; cell line R366.4) have been described before to show a spatially ordered process of epithelial-mesenchymal transition in vitro. In the present investigations, we have studied variables of culturing conditions which influence the reproducibility of the formation of crater-like ingression centers in the colonies. Critical parameters are found to be age and density of mouse embryonic fibroblast (MEF) feeder cell layers, the mode of mitotic inactivation of the MEFs (mitomycin C, or irradiation), and the mode of rhESC isolation during subculturing (enzymatic/mechanical cell cluster isolation; type of enzyme). The described culturing system appears to offer a reproducible in vitro model potentially useful for studies on cellular processes involved in gastrulation in the primate. The Society for In Vitro Biology, 2010 Embryonic stem cells nationallicence Epithelial-mesenchymal transition nationallicence Gastrulation nationallicence Ingression centers nationallicence Feeder cell layers nationallicence Maranca-Hüwel Birgit Institut für Anatomie, Universität Duisburg-Essen, Universitätsklinikum, Hufelandstr. 55, 45122, Essen, Germany aut Denker Hans-Werner Institut für Anatomie, Universität Duisburg-Essen, Universitätsklinikum, Hufelandstr. 55, 45122, Essen, Germany aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/6(2010-06-01), 516-528 1071-2690 46:6<516 2010 46 11626 https://doi.org/10.1007/s11626-010-9272-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9272-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maranca-Hüwel Birgit Institut für Anatomie, Universität Duisburg-Essen, Universitätsklinikum, Hufelandstr. 55, 45122, Essen, Germany aut NATIONALLICENCE 700 1- Denker Hans-Werner Institut für Anatomie, Universität Duisburg-Essen, Universitätsklinikum, Hufelandstr. 55, 45122, Essen, Germany aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/6(2010-06-01), 516-528 1071-2690 46:6<516 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer