caa a22 4500 445292407 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20100601xx s 000 0 eng 10.1007/s11626-010-9274-6 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9274-6 Side populations of glioblastoma cells are less sensitive to HSV-TK/GCV suicide gene therapy system than the non-side population [Elektronische Daten] [Weiwei Hu, Weiguo Liu] Side populations of glioblastoma cells are resistant to chemotherapy basically due to ABCG2-mediated efflux of small-molecule drugs. The herpes simplex virus thymidine kinase/ganciclovir suicide gene therapy system is one of the best-characterized strategies for malignant tumors including glioblastoma. Since this system involves a small-molecule drug ganciclovir, we wonder if glioblastoma side population cells are able to "pump out” ganciclovir and thus resistant to this suicide gene therapy. By 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay, we found that side populations are more resistant to this system than non-side populations. By flow cytometry and competition assay, we found that ganciclovir is a substrate for ABCG2. The Society for In Vitro Biology, 2010 Side population nationallicence Glioblastoma nationallicence Ganciclovir nationallicence Suicide gene therapy nationallicence Hu Weiwei Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, No. 88 Jiefang Road, 310009, Hangzhou, China aut Liu Weiguo Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, No. 88 Jiefang Road, 310009, Hangzhou, China aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/6(2010-06-01), 497-501 1071-2690 46:6<497 2010 46 11626 https://doi.org/10.1007/s11626-010-9274-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9274-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Weiwei Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, No. 88 Jiefang Road, 310009, Hangzhou, China aut NATIONALLICENCE 700 1- Liu Weiguo Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, No. 88 Jiefang Road, 310009, Hangzhou, China aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/6(2010-06-01), 497-501 1071-2690 46:6<497 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer