caa a22 4500 445292415 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20100601xx s 000 0 eng 10.1007/s11626-010-9273-7 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9273-7 New aspects in the synthesis and secretion of lysozyme by cultured human monocyte cell lines [Elektronische Daten] [Racha Helal, Matthias Melzig] The main purpose of this study was to investigate lysozyme synthesis and secretion in three human monocyte cell lines: U-937, HL-60, and THP-1, using sensitive fluorescence-based assay of lysozyme activity. PMA and hIFN-γ were evaluated for inducing lysozyme activity. Using well-defined cell lines from the cell culture collection, no lysozyme activity could be detected in the cultured U-937 cells either with or without addition of the inducing factors. These data suggested, contrary to previous reports, that U-937 cell line cannot synthesize or secrete active lysozyme. THP-1 and HL-60 cells were proved to produce enzymatically active lysozyme in increasing amounts with the time course. PMA and hIFN-γ had no significant inducing effect on the production or the release of active lysozyme in THP-1 and HL-60 cells. We showed inhibiting effect of PMA and hIFN-γ on the lysozyme activity, particularly in HL-60 cell line. The Society for In Vitro Biology, 2010 U-937 nationallicence HL-60 nationallicence THP-1 nationallicence Fluorescence-based lysozyme activity assay nationallicence PMA nationallicence Human interferon-γ nationallicence Helal Racha Department of Pharrmaceutical Biology, Institute of Pharmacy, Free University of Berlin, Königin-Luise-Str. 2+4, 14195, Berlin, Germany aut Melzig Matthias Department of Pharrmaceutical Biology, Institute of Pharmacy, Free University of Berlin, Königin-Luise-Str. 2+4, 14195, Berlin, Germany aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/6(2010-06-01), 492-496 1071-2690 46:6<492 2010 46 11626 https://doi.org/10.1007/s11626-010-9273-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9273-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Helal Racha Department of Pharrmaceutical Biology, Institute of Pharmacy, Free University of Berlin, Königin-Luise-Str. 2+4, 14195, Berlin, Germany aut NATIONALLICENCE 700 1- Melzig Matthias Department of Pharrmaceutical Biology, Institute of Pharmacy, Free University of Berlin, Königin-Luise-Str. 2+4, 14195, Berlin, Germany aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/6(2010-06-01), 492-496 1071-2690 46:6<492 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer