caa a22 4500 445292423 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20100601xx s 000 0 eng 10.1007/s11626-009-9270-x doi (NATIONALLICENCE)springer-10.1007/s11626-009-9270-x Regulation of the expression balance of angiopoietin-1 and angiopoietin-2 by Shh and FGF-2 [Elektronische Daten] [Takaaki Fujii, Hiroyuki Kuwano] Sonic hedgehog (Shh) is a typical morphogen to regulate epithelial-mesenchymal interactions during embryonic development. Shh is also an indirect angiogenic agent upregulating other angiogenic factors, including angiopoietin-1 (Ang-1). Recent studies revealed that angiogenesis induced by Shh is characterized by distinct large-diameter vessels with less branching. Ang-1 promotes blood vessel maturation, and angiopoietin-2 (Ang-2) counteracts Ang-1 activity and regulates vascular branching. Thus, we hypothesized that Shh-induced angiogenesis is affected by expression of Ang-1 and Ang-2, and we investigated the regulatory system of angiopoietins by Shh in vitro. Shh enhanced Ang-1 expression but did not enhance vascular endothelial growth factor in fibroblasts. The upregulation of Ang-1 expression by Shh was significantly decreased by fibroblast growth factor-2 (FGF-2), a potent angiogenic factor. Furthermore, FGF-2 increased the expression of Ang-2 in endothelial cells. These findings suggest that Shh and FGF-2 regulate the expression balance of vascular morphogens Ang-1 and Ang-2 and are involved in angiogenesis. The Society for In Vitro Biology, 2010 Shh nationallicence FGF-2 nationallicence Angiopoietin-1 nationallicence Angiopoietin-2 nationallicence Angiogenesis nationallicence Fujii Takaaki Department of General Surgical Science, Graduate School of Medicine, Gunma University, 371-8511, 3-39-15 Showa-machi, Maebashi, Gunma, Japan aut Kuwano Hiroyuki Department of General Surgical Science, Graduate School of Medicine, Gunma University, 371-8511, 3-39-15 Showa-machi, Maebashi, Gunma, Japan aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/6(2010-06-01), 487-491 1071-2690 46:6<487 2010 46 11626 https://doi.org/10.1007/s11626-009-9270-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9270-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fujii Takaaki Department of General Surgical Science, Graduate School of Medicine, Gunma University, 371-8511, 3-39-15 Showa-machi, Maebashi, Gunma, Japan aut NATIONALLICENCE 700 1- Kuwano Hiroyuki Department of General Surgical Science, Graduate School of Medicine, Gunma University, 371-8511, 3-39-15 Showa-machi, Maebashi, Gunma, Japan aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/6(2010-06-01), 487-491 1071-2690 46:6<487 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer