caa a22 4500 44529244X CHVBK 20180317142532.0 cr unu---uuuuu 170323e20101001xx s 000 0 eng 10.1007/s11626-010-9332-0 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9332-0 Micromanipulation of culture niche permits long-term expansion of dental pulp stem cells—an economic and commercial angle [Elektronische Daten] [Vijayendran Govindasamy, Veronica Ronald, Swapnil Totey, Salina Binti Din, Wan Mustafa, Satish Totey, Zubaidah Zakaria, Ramesh Bhonde] Stem cells isolated from dental pulp possess the capacity for self-renewal and the potential for multi-lineage differentiation. However, dental pulp stem cells have different characteristics in terms of their culture conditions. The success of stem cells culture is governed by its micro-environmental niche. Therefore, we studied the effects of culture niche on long-term expansion of dental pulp stem cells in terms of cell morphology, growth kinetics, senescence pattern, cell surface marker expression differentiation capacity, and seeding plating density of dental pulp stem cells in four different, widely used media composition Among the various basal media tested, α-minimum essential media and knock out-minimum essential media supplemented with 10% fetal bovine serum were found to be the most optimal media composition in preserving the phenotypic characteristics and differentiation potential for prolonged periods as compared with DMEM-F12 and DMEM-LG. Plating density has been shown to affect overall yield. As a conclusion, the adoption of an appropriate culture system significantly improved cell yield, thus enabling the attainment of sufficient yields for therapeutic applications economizing in terms of cost of production and minimizing seeding cell density for maximum yield. The Society for In Vitro Biology, 2010 Dental pulp nationallicence Mesenchymal stromal cell nationallicence Optimum culture conditions nationallicence Differentiation nationallicence Govindasamy Vijayendran Stempeutics Research Malaysia, Sdn Bhd (773817-K) Lot 3-i-7, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000, Kuala Lumpur, Malaysia aut Ronald Veronica Cancer Research Center, Institute for Medical Research (IMR), Jalan Pahang, 50588, Kuala Lumpur, Malaysia aut Totey Swapnil Stempeutics Research Malaysia, Sdn Bhd (773817-K) Lot 3-i-7, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000, Kuala Lumpur, Malaysia aut Binti Din Salina Department of Oral Surgery, Hospital Kuala Lumpur, Kuala Lumpur, Malaysia aut Mustafa Wan Department of Oral Surgery, Hospital Kuala Lumpur, Kuala Lumpur, Malaysia aut Totey Satish Stempeutics Research Malaysia, Sdn Bhd (773817-K) Lot 3-i-7, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000, Kuala Lumpur, Malaysia aut Zakaria Zubaidah Cancer Research Center, Institute for Medical Research (IMR), Jalan Pahang, 50588, Kuala Lumpur, Malaysia aut Bhonde Ramesh Stempeutics Research Malaysia, Sdn Bhd (773817-K) Lot 3-i-7, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000, Kuala Lumpur, Malaysia aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/9(2010-10-01), 764-773 1071-2690 46:9<764 2010 46 11626 https://doi.org/10.1007/s11626-010-9332-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9332-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Govindasamy Vijayendran Stempeutics Research Malaysia, Sdn Bhd (773817-K) Lot 3-i-7, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000, Kuala Lumpur, Malaysia aut NATIONALLICENCE 700 1- Ronald Veronica Cancer Research Center, Institute for Medical Research (IMR), Jalan Pahang, 50588, Kuala Lumpur, Malaysia aut NATIONALLICENCE 700 1- Totey Swapnil Stempeutics Research Malaysia, Sdn Bhd (773817-K) Lot 3-i-7, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000, Kuala Lumpur, Malaysia aut NATIONALLICENCE 700 1- Binti Din Salina Department of Oral Surgery, Hospital Kuala Lumpur, Kuala Lumpur, Malaysia aut NATIONALLICENCE 700 1- Mustafa Wan Department of Oral Surgery, Hospital Kuala Lumpur, Kuala Lumpur, Malaysia aut NATIONALLICENCE 700 1- Totey Satish Stempeutics Research Malaysia, Sdn Bhd (773817-K) Lot 3-i-7, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000, Kuala Lumpur, Malaysia aut NATIONALLICENCE 700 1- Zakaria Zubaidah Cancer Research Center, Institute for Medical Research (IMR), Jalan Pahang, 50588, Kuala Lumpur, Malaysia aut NATIONALLICENCE 700 1- Bhonde Ramesh Stempeutics Research Malaysia, Sdn Bhd (773817-K) Lot 3-i-7, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000, Kuala Lumpur, Malaysia aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/9(2010-10-01), 764-773 1071-2690 46:9<764 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer