caa a22 4500 445292458 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20101001xx s 000 0 eng 10.1007/s11626-010-9344-9 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9344-9 Cultured human corneal epithelial stem/progenitor cells derived from the corneal limbus [Elektronische Daten] [Naoki Yamamoto, Koji Hirano, Hajime Kojima, Mariko Sumitomo, Hiromi Yamashita, Masahiko Ayaki, Koki Taniguchi, Atsuhiro Tanikawa, Masayuki Horiguchi] Stem/progenitor cells of the human corneal epithelium are present in the human corneal limbus, and several corneal epithelial stem/progenitor cell markers have been reported. Recently, the neurotrophin family receptors were reported to be useful markers of corneal epithelial stem/progenitor cells. Therefore, we examined an enzymatic separation method for obtaining corneal epithelial stem/progenitor cells and measuring the change in the expression of low-affinity neurotrophin receptor p75 (p75NTR), a receptor belonging to the neurotrophin family. As a result, it was found that our separation method preserved cell viability. Furthermore, p75NTR was mainly observed in epithelial basal cells as were the corneal epithelial stem/progenitor markers p63 and integrin β1. p75NTR was also observed in the cultured cells, but its frequency decreased with passage. In conclusion, we propose that our culture method will enable the culture of corneal stem cells and that it is a useful tool for elucidating the molecular basis of the niche that is necessary for the maintenance of epithelial stem cells in the corneal limbus. Furthermore, we conclude that p75NTR is a useful cell marker for evaluating the characteristics of stem/progenitor cells in culture. The Society for In Vitro Biology, 2010 Human corneal epithelium nationallicence Corneal limbus nationallicence Low-affinity neurotrophin receptor p75 (p75NTR) nationallicence p63 nationallicence Integrin β1 nationallicence Yamamoto Naoki Laboratory of Molecular Biology and Histochemistry, Fujita Health University Joint Research Laboratory, 1-98 Dengakugakubo, Kutsukake-cho, 470-1192, Toyoake, Aichi, Japan aut Hirano Koji Department of Ophthalmology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, 470-1192, Toyoake, Aichi, Japan aut Kojima Hajime Division of Pharmacology, Biological Safety Research Center, Japanese Center for the Validation of Alternative Methods (JaCVAM), National Institute of Health Sciences, 1-18-1, Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut Sumitomo Mariko Laboratory of Molecular Biology and Histochemistry, Fujita Health University Joint Research Laboratory, 1-98 Dengakugakubo, Kutsukake-cho, 470-1192, Toyoake, Aichi, Japan aut Yamashita Hiromi Laboratory of Molecular Biology and Histochemistry, Fujita Health University Joint Research Laboratory, 1-98 Dengakugakubo, Kutsukake-cho, 470-1192, Toyoake, Aichi, Japan aut Ayaki Masahiko Department of Ophthalmology, Showa University Fujigaoka Rehabilitation Hospital, 2-1-1, Fujigaoka, Aoba-ku, 227-8518, Yokohama, Kanagawa, Japan aut Taniguchi Koki Laboratory of Molecular Biology and Histochemistry, Fujita Health University Joint Research Laboratory, 1-98 Dengakugakubo, Kutsukake-cho, 470-1192, Toyoake, Aichi, Japan aut Tanikawa Atsuhiro Department of Ophthalmology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, 470-1192, Toyoake, Aichi, Japan aut Horiguchi Masayuki Department of Ophthalmology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, 470-1192, Toyoake, Aichi, Japan aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/9(2010-10-01), 774-780 1071-2690 46:9<774 2010 46 11626 https://doi.org/10.1007/s11626-010-9344-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9344-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamamoto Naoki Laboratory of Molecular Biology and Histochemistry, Fujita Health University Joint Research Laboratory, 1-98 Dengakugakubo, Kutsukake-cho, 470-1192, Toyoake, Aichi, Japan aut NATIONALLICENCE 700 1- Hirano Koji Department of Ophthalmology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, 470-1192, Toyoake, Aichi, Japan aut NATIONALLICENCE 700 1- Kojima Hajime Division of Pharmacology, Biological Safety Research Center, Japanese Center for the Validation of Alternative Methods (JaCVAM), National Institute of Health Sciences, 1-18-1, Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Sumitomo Mariko Laboratory of Molecular Biology and Histochemistry, Fujita Health University Joint Research Laboratory, 1-98 Dengakugakubo, Kutsukake-cho, 470-1192, Toyoake, Aichi, Japan aut NATIONALLICENCE 700 1- Yamashita Hiromi Laboratory of Molecular Biology and Histochemistry, Fujita Health University Joint Research Laboratory, 1-98 Dengakugakubo, Kutsukake-cho, 470-1192, Toyoake, Aichi, Japan aut NATIONALLICENCE 700 1- Ayaki Masahiko Department of Ophthalmology, Showa University Fujigaoka Rehabilitation Hospital, 2-1-1, Fujigaoka, Aoba-ku, 227-8518, Yokohama, Kanagawa, Japan aut NATIONALLICENCE 700 1- Taniguchi Koki Laboratory of Molecular Biology and Histochemistry, Fujita Health University Joint Research Laboratory, 1-98 Dengakugakubo, Kutsukake-cho, 470-1192, Toyoake, Aichi, Japan aut NATIONALLICENCE 700 1- Tanikawa Atsuhiro Department of Ophthalmology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, 470-1192, Toyoake, Aichi, Japan aut NATIONALLICENCE 700 1- Horiguchi Masayuki Department of Ophthalmology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, 470-1192, Toyoake, Aichi, Japan aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/9(2010-10-01), 774-780 1071-2690 46:9<774 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer