caa a22 4500 445292466 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20101001xx s 000 0 eng 10.1007/s11626-010-9337-8 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9337-8 Characterization of human embryonic stem cell-derived hematopoietic progenitor phenotype [Elektronische Daten] [Hai-Yang Zhou, Xiao-Yi Yang, Li-Jian Tao, Yue-Tian Chang, Ru-Ping Dai] Differentiation of human embryonic stem cells (hESCs) into hematopoietic lineages using various methods has been reported. However, the phenotype that precisely defines the hematopoietic progenitor compartment with clonogenic activities has yet to be determined. Here, we measured and characterized progenitor function of subfractions of cells prospectively isolated from human embryoid bodies (hEBs) during hematopoietic differentiation basing on surface markers CD45, CD34, CD43, and CD38. We report that hematopoietic progenitors predominantly resided in the CD45+ subset. CD43+ cells lacking CD45 expression were largely devoid of progenitor activity. However, progenitor activity and multipotentiality was more enriched in CD45+ cells co-expressing CD43. CD45+ subset co-expressing CD34 but lacking CD38 expression (CD45+CD34+CD38-) were further enriched for CFU capacity compared to the CD45+CD34+CD38+ subset. Our study demonstrates a role of CD43 in enriching hematopoietic progenitors derived from hEBs and reveals a hierarchical organization of hESC-derived hematopoietic progenitor compartments defined by phenotypic markers. The Society for In Vitro Biology, 2010 Colony-forming unit assay nationallicence Flow cytometry nationallicence Hematopoietic differentiation nationallicence Human embryonic stem cells nationallicence Zhou Hai-Yang Department of Anesthesia, The Second Xiangya Hospital of Central South University, Ren-Min Road no. 139, 410011, Changsha, Hunan, China aut Yang Xiao-Yi Reproductive & Genetic Specialized Hospital, Xiangya Medical College, Central South University, Changsha, Hunan, China aut Tao Li-Jian Department of Medicine, Xiangya Hospital of Central South University, Changsha, Hunan, China aut Chang Yue-Tian Department of Anesthesia, The Second Xiangya Hospital of Central South University, Ren-Min Road no. 139, 410011, Changsha, Hunan, China aut Dai Ru-Ping Department of Anesthesia, The Second Xiangya Hospital of Central South University, Ren-Min Road no. 139, 410011, Changsha, Hunan, China aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/9(2010-10-01), 733-737 1071-2690 46:9<733 2010 46 11626 https://doi.org/10.1007/s11626-010-9337-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9337-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Hai-Yang Department of Anesthesia, The Second Xiangya Hospital of Central South University, Ren-Min Road no. 139, 410011, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Yang Xiao-Yi Reproductive & Genetic Specialized Hospital, Xiangya Medical College, Central South University, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Tao Li-Jian Department of Medicine, Xiangya Hospital of Central South University, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Chang Yue-Tian Department of Anesthesia, The Second Xiangya Hospital of Central South University, Ren-Min Road no. 139, 410011, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Dai Ru-Ping Department of Anesthesia, The Second Xiangya Hospital of Central South University, Ren-Min Road no. 139, 410011, Changsha, Hunan, China aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/9(2010-10-01), 733-737 1071-2690 46:9<733 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer