caa a22 4500 445292474 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20101001xx s 000 0 eng 10.1007/s11626-010-9345-8 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9345-8 Morphological and biological characterization of cell line developed from bovine Echinococcus granulosus [Elektronische Daten] [Claudia Echeverría, Dora Isolabella, Elio Gonzalez, Araceli Leonardelli, Laura Prada, Alina Perrone, Alicia Fuchs] The taeniid tapeworm Echinococcus granulosus is the causative agent of echinococcal disease, a major zoonosis with worldwide distribution. Several efforts to establish an in vitro model of E. granulosus have been undertaken; however, many of them have been designed for Echinococcus multilocularis. In the present study, we have described and characterized a stable cell line obtained from E. granulosus bovine protoscoleces maintained 3yr in vitro. Growth characterization, morphology by light, fluorescent and electronic microscopy, and karyotyping were carried out. Cell culture origin was confirmed by immunofluorescent detection of AgB4 antigen and by PCR for the mitochondrial cytochrome c-oxidase subunit 1 (DCO1) gene. Cells seeded in agarose biphasic culture resembled a cystic structure, similar to the one formed in secondary hosts. This cell line could be a useful tool to research equinococcal behavior, allowing additional physiological and pharmacological studies, such as the effect of growth factors, nutrients, and antiparasitic drugs on cell viability and growth and on cyst formation. The Society for In Vitro Biology, 2010 Echinococcus granulosus nationallicence Cell line characterization nationallicence Cyst formation nationallicence Echeverría Claudia Centro de Altos Estudios en Ciencias de la Salud (CAECIS), Universidad Abierta Interamericana (UAI), Avenida Montes de Oca 745, 1270, Buenos Aires, Argentina aut Isolabella Dora Departamento de Farmacología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, C1121ABG, Buenos Aires, Argentina aut Gonzalez Elio Centro de Altos Estudios en Ciencias de la Salud (CAECIS), Universidad Abierta Interamericana (UAI), Avenida Montes de Oca 745, 1270, Buenos Aires, Argentina aut Leonardelli Araceli Centro de Altos Estudios en Ciencias de la Salud (CAECIS), Universidad Abierta Interamericana (UAI), Avenida Montes de Oca 745, 1270, Buenos Aires, Argentina aut Prada Laura Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, C1121ABG, Buenos Aires, Argentina aut Perrone Alina Centro de Altos Estudios en Ciencias de la Salud (CAECIS), Universidad Abierta Interamericana (UAI), Avenida Montes de Oca 745, 1270, Buenos Aires, Argentina aut Fuchs Alicia Centro de Altos Estudios en Ciencias de la Salud (CAECIS), Universidad Abierta Interamericana (UAI), Avenida Montes de Oca 745, 1270, Buenos Aires, Argentina aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/9(2010-10-01), 781-792 1071-2690 46:9<781 2010 46 11626 https://doi.org/10.1007/s11626-010-9345-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9345-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Echeverría Claudia Centro de Altos Estudios en Ciencias de la Salud (CAECIS), Universidad Abierta Interamericana (UAI), Avenida Montes de Oca 745, 1270, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Isolabella Dora Departamento de Farmacología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, C1121ABG, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Gonzalez Elio Centro de Altos Estudios en Ciencias de la Salud (CAECIS), Universidad Abierta Interamericana (UAI), Avenida Montes de Oca 745, 1270, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Leonardelli Araceli Centro de Altos Estudios en Ciencias de la Salud (CAECIS), Universidad Abierta Interamericana (UAI), Avenida Montes de Oca 745, 1270, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Prada Laura Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, C1121ABG, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Perrone Alina Centro de Altos Estudios en Ciencias de la Salud (CAECIS), Universidad Abierta Interamericana (UAI), Avenida Montes de Oca 745, 1270, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Fuchs Alicia Centro de Altos Estudios en Ciencias de la Salud (CAECIS), Universidad Abierta Interamericana (UAI), Avenida Montes de Oca 745, 1270, Buenos Aires, Argentina aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/9(2010-10-01), 781-792 1071-2690 46:9<781 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer