caa a22 4500 445292490 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20101001xx s 000 0 eng 10.1007/s11626-010-9333-z doi (NATIONALLICENCE)springer-10.1007/s11626-010-9333-z Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues [Elektronische Daten] [Rita Barallon, Steven Bauer, John Butler, Amanda Capes-Davis, Wilhelm Dirks, Eugene Elmore, Manohar Furtado, Margaret Kline, Arihiro Kohara, Georgyi Los, Roderick MacLeod, John Masters, Mark Nardone, Roland Nardone, Raymond Nims, Paul Price, Yvonne Reid, Jaiprakash Shewale, Gregory Sykes, Anton Steuer, Douglas Storts, Jim Thomson, Zenobia Taraporewala, Christine Alston-Roberts, Liz Kerrigan] Cell misidentification and cross-contamination have plagued biomedical research for as long as cells have been employed as research tools. Examples of misidentified cell lines continue to surface to this day. Efforts to eradicate the problem by raising awareness of the issue and by asking scientists voluntarily to take appropriate actions have not been successful. Unambiguous cell authentication is an essential step in the scientific process and should be an inherent consideration during peer review of papers submitted for publication or during review of grants submitted for funding. In order to facilitate proper identity testing, accurate, reliable, inexpensive, and standardized methods for authentication of cells and cell lines must be made available. To this end, an international team of scientists is, at this time, preparing a consensus standard on the authentication of human cells using short tandem repeat (STR) profiling. This standard, which will be submitted for review and approval as an American National Standard by the American National Standards Institute, will provide investigators guidance on the use of STR profiling for authenticating human cell lines. Such guidance will include methodological detail on the preparation of the DNA sample, the appropriate numbers and types of loci to be evaluated, and the interpretation and quality control of the results. Associated with the standard itself will be the establishment and maintenance of a public STR profile database under the auspices of the National Center for Biotechnology Information. The consensus standard is anticipated to be adopted by granting agencies and scientific journals as appropriate methodology for authenticating human cell lines, stem cells, and tissues. The Author(s), 2010 Cell authentication nationallicence STR profiling nationallicence Consensus standard nationallicence Quality control nationallicence Barallon Rita LGC, Queens Road, TW11 0LY, Teddington, Middlesex, UK aut Bauer Steven Division of Cellular and Gene Therapies, Office of Cellular, Tissue and Gene Therapies, FDA/Center for Biologics Evaluation & Research, NIH Bldg 29B 2NN10 HFM-740, 8800 Rockville Pike, 20892, Bethesda, MD, USA aut Butler John Biochemical Science Division (831), Advanced Chemical Science Laboratory (227), National Institutes of Standards and Technology, Room B226, 100 Bureau Drive, Stop 8312, 20899-8312, Gaithersburg, MD, USA aut Capes-Davis Amanda CellBank Australia, Children's Medical Research Institute, Westmead, New South Wales, Australia aut Dirks Wilhelm DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7b, 38124, Braunschweig, Germany aut Elmore Eugene Department of Radiation Oncology, University of California, Medical Sciences I, B146D, 92697, Irvine, CA, USA aut Furtado Manohar Applied Markets/Genetic Systems Life Technologies, 850 Lincoln Centre Drive, 94404, Foster City, CA, USA aut Kline Margaret Biochemical Science Division (831), Advanced Chemical Science Laboratory (227), National Institutes of Standards and Technology, Room B226, 100 Bureau Drive, Stop 8312, 20899-8312, Gaithersburg, MD, USA aut Kohara Arihiro Department Biomedical Resources, Laboratory of Cell Cultures, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, 567-0085, Ibaraki, Osaka, Japan aut Los Georgyi Neuroscience Training Program, University of Wisconsin-Madison, 1300 University Av., WI, Madison, USA aut MacLeod Roderick DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7b, 38124, Braunschweig, Germany aut Masters John Institute of Urology, University College London, 67 Riding House Street, W1W 7EJ, London, UK aut Nardone Mark Bio-Trac Program, The Foundation for the Advanced Education in the Sciences at the National Institutes of Health, 20892, Bethesda, MD, USA aut Nardone Roland Catholic University of America, Cell and Molecular Biology, 620 Michigan Ave NE, 20064, Washington, DC, USA aut Nims Raymond RMC Pharmaceutical Solutions, Inc., 2150 Miller Drive, Suite A, 80501, Longmont, CO, USA aut Price Paul D-Finitive Cell Technology, Room B-33, 1023 Wappoo Rd, 29407, Charleston, SC, USA aut Reid Yvonne American Type Culture Collection, 10801 University Blvd., 20110, Manassas, VA, USA aut Shewale Jaiprakash Applied Markets/Genetic Systems Life Technologies, 850 Lincoln Centre Drive, 94404, Foster City, CA, USA aut Sykes Gregory American Type Culture Collection, 10801 University Blvd., 20110, Manassas, VA, USA aut Steuer Anton BioReliance, 14920 Broschart Road, 20850, Rockville, MD, USA aut Storts Douglas Nucleic Acid Technologies, Promega Corporation, 2800 Woods Hollow Road, 53711, Madison, WI, USA aut Thomson Jim LGC, Queens Road, TW11 0LY, Teddington, Middlesex, UK aut Taraporewala Zenobia Division of Cellular and Gene Therapies, Office of Cellular, Tissue, and Gene Therapies, FDA/Center for Biologics Evaluation and Research, 1401 Rockville Pike, Room 200N, 20852, Rockville, MD, USA aut Alston-Roberts Christine American Type Culture Collection, 10801 University Blvd., 20110, Manassas, VA, USA aut Kerrigan Liz American Type Culture Collection, 10801 University Blvd., 20110, Manassas, VA, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/9(2010-10-01), 727-732 1071-2690 46:9<727 2010 46 11626 https://doi.org/10.1007/s11626-010-9333-z text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9333-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barallon Rita LGC, Queens Road, TW11 0LY, Teddington, Middlesex, UK aut NATIONALLICENCE 700 1- Bauer Steven Division of Cellular and Gene Therapies, Office of Cellular, Tissue and Gene Therapies, FDA/Center for Biologics Evaluation & Research, NIH Bldg 29B 2NN10 HFM-740, 8800 Rockville Pike, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Butler John Biochemical Science Division (831), Advanced Chemical Science Laboratory (227), National Institutes of Standards and Technology, Room B226, 100 Bureau Drive, Stop 8312, 20899-8312, Gaithersburg, MD, USA aut NATIONALLICENCE 700 1- Capes-Davis Amanda CellBank Australia, Children's Medical Research Institute, Westmead, New South Wales, Australia aut NATIONALLICENCE 700 1- Dirks Wilhelm DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7b, 38124, Braunschweig, Germany aut NATIONALLICENCE 700 1- Elmore Eugene Department of Radiation Oncology, University of California, Medical Sciences I, B146D, 92697, Irvine, CA, USA aut NATIONALLICENCE 700 1- Furtado Manohar Applied Markets/Genetic Systems Life Technologies, 850 Lincoln Centre Drive, 94404, Foster City, CA, USA aut NATIONALLICENCE 700 1- Kline Margaret Biochemical Science Division (831), Advanced Chemical Science Laboratory (227), National Institutes of Standards and Technology, Room B226, 100 Bureau Drive, Stop 8312, 20899-8312, Gaithersburg, MD, USA aut NATIONALLICENCE 700 1- Kohara Arihiro Department Biomedical Resources, Laboratory of Cell Cultures, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, 567-0085, Ibaraki, Osaka, Japan aut NATIONALLICENCE 700 1- Los Georgyi Neuroscience Training Program, University of Wisconsin-Madison, 1300 University Av., WI, Madison, USA aut NATIONALLICENCE 700 1- MacLeod Roderick DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7b, 38124, Braunschweig, Germany aut NATIONALLICENCE 700 1- Masters John Institute of Urology, University College London, 67 Riding House Street, W1W 7EJ, London, UK aut NATIONALLICENCE 700 1- Nardone Mark Bio-Trac Program, The Foundation for the Advanced Education in the Sciences at the National Institutes of Health, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Nardone Roland Catholic University of America, Cell and Molecular Biology, 620 Michigan Ave NE, 20064, Washington, DC, USA aut NATIONALLICENCE 700 1- Nims Raymond RMC Pharmaceutical Solutions, Inc., 2150 Miller Drive, Suite A, 80501, Longmont, CO, USA aut NATIONALLICENCE 700 1- Price Paul D-Finitive Cell Technology, Room B-33, 1023 Wappoo Rd, 29407, Charleston, SC, USA aut NATIONALLICENCE 700 1- Reid Yvonne American Type Culture Collection, 10801 University Blvd., 20110, Manassas, VA, USA aut NATIONALLICENCE 700 1- Shewale Jaiprakash Applied Markets/Genetic Systems Life Technologies, 850 Lincoln Centre Drive, 94404, Foster City, CA, USA aut NATIONALLICENCE 700 1- Sykes Gregory American Type Culture Collection, 10801 University Blvd., 20110, Manassas, VA, USA aut NATIONALLICENCE 700 1- Steuer Anton BioReliance, 14920 Broschart Road, 20850, Rockville, MD, USA aut NATIONALLICENCE 700 1- Storts Douglas Nucleic Acid Technologies, Promega Corporation, 2800 Woods Hollow Road, 53711, Madison, WI, USA aut NATIONALLICENCE 700 1- Thomson Jim LGC, Queens Road, TW11 0LY, Teddington, Middlesex, UK aut NATIONALLICENCE 700 1- Taraporewala Zenobia Division of Cellular and Gene Therapies, Office of Cellular, Tissue, and Gene Therapies, FDA/Center for Biologics Evaluation and Research, 1401 Rockville Pike, Room 200N, 20852, Rockville, MD, USA aut NATIONALLICENCE 700 1- Alston-Roberts Christine American Type Culture Collection, 10801 University Blvd., 20110, Manassas, VA, USA aut NATIONALLICENCE 700 1- Kerrigan Liz American Type Culture Collection, 10801 University Blvd., 20110, Manassas, VA, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/9(2010-10-01), 727-732 1071-2690 46:9<727 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer