caa a22 4500 445292512 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20101001xx s 000 0 eng 10.1007/s11626-010-9335-x doi (NATIONALLICENCE)springer-10.1007/s11626-010-9335-x Schwann-like cells can be induction from human nestin-positive amniotic fluid mesenchymal stem cells [Elektronische Daten] [Tai-Mao Jiang, Zhi-Jun Yang, Chui-Ze Kong, Hong-Tian Zhang] We examined the morphological, phenotypic, and functional characteristics of human amniotic fluid mesenchymal stem cells (AF-MSCs) differentiated towards a Schwann cell lineage. Initially, we induced human AF-MSCs into nestin-positive AF-MSCs. And then, these nestin-positive AF-MSCs were induced into floating neurospheres. After that, neurospheres were induced to differentiate into Schwann-like cells using glia growth factors. In comparison with AF-MSCs, nestin-positive AF-MSCs significantly increased the ratio of neurosphere formation and the percentage of nestin expression in the neurosphere. Differentiated AF-MSCs showed morphological changes similar to those found in Schwann cells. Expression of the Schwann cell markers was determined by immunocytochemical staining and western blotting. Furthermore, differentiated AF-MSCs could promote neurite outgrowth in co-culture with dorsal root ganglia neurons. These results suggest that conversion of human nestin-positive AF-MSCs into cells with Schwann-like cell characteristics is possible and that these cells may have the potential for future cellular therapy for peripheral neurological disorders. The Society for In Vitro Biology, 2010 Amniotic fluid mesenchymal stem cells nationallicence Transdifferentiation nationallicence Schwann cell nationallicence Jiang Tai-Mao Department of Urology, The First Affiliated Hospital, China Medical University, 100039, Shenyang, China aut Yang Zhi-Jun Department of Neurosurgery, The Military General Hospital of Beijing PLA, 100700, Beijing, China aut Kong Chui-Ze Department of Urology, The First Affiliated Hospital, China Medical University, 100039, Shenyang, China aut Zhang Hong-Tian Department of Urology, The First Affiliated Hospital, China Medical University, 100039, Shenyang, China aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/9(2010-10-01), 793-800 1071-2690 46:9<793 2010 46 11626 https://doi.org/10.1007/s11626-010-9335-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9335-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jiang Tai-Mao Department of Urology, The First Affiliated Hospital, China Medical University, 100039, Shenyang, China aut NATIONALLICENCE 700 1- Yang Zhi-Jun Department of Neurosurgery, The Military General Hospital of Beijing PLA, 100700, Beijing, China aut NATIONALLICENCE 700 1- Kong Chui-Ze Department of Urology, The First Affiliated Hospital, China Medical University, 100039, Shenyang, China aut NATIONALLICENCE 700 1- Zhang Hong-Tian Department of Urology, The First Affiliated Hospital, China Medical University, 100039, Shenyang, China aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/9(2010-10-01), 793-800 1071-2690 46:9<793 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer