caa a22 4500 445292520 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20101001xx s 000 0 eng 10.1007/s11626-010-9331-1 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9331-1 Neuronal progenitor cells seeded in fibrin gel differentiate into ChAT-positive neuron [Elektronische Daten] [Jinbo Liu, Zhijian Zhang, Aihua Gong, Xudong Cao, Leimin Qian, Lirong Duan, Xianglan Sun, Xuefeng Bu] Previous studies indicate that neural progenitor cells seeded in fibrin can differentiate into the glia cells and neurons. However, whether fibrin gel can induce differentiation of neural progenitor cells of spinal cord into motoneurons remains to be elucidated. In this study, we prepared a fibrin-based hybrid gel incorporated with laminin and fibronectin using rat fresh plasma as a crosslinking agent. The physical properties of this hybrid gel were examined with electron microscope, as well as its degradation and vascularization after subcutaneous transplantation with histological methods. Immunofluorescence stainings were used to study the proliferation and differentiation of the neural progenitor cells seeded in this 3D hybrid gel. The results indicate that such a hybrid gel possesses the unique physical characteristics, and the ability to promote both the proliferation and differentiation of the neural progenitor cells into neuron, including ChAT-positive motoneuron. Thus, it is suggested that this hybrid gel might be suitable for a mimetic tissue transplant for central nerve system injury, such as injured spinal cord repair. The Society for In Vitro Biology, 2010 Fibrin-based gel nationallicence Neural progenitor cell nationallicence ChAT nationallicence Neuron nationallicence Liu Jinbo Department of Orthopedics, The Third Hospital Affiliated to Suzhou University, 213003, Changzhou, China aut Zhang Zhijian School of Medicine, Jiangsu University, 212013, Zhenjiang, Jiangsu, China aut Gong Aihua School of Medicine, Jiangsu University, 212013, Zhenjiang, Jiangsu, China aut Cao Xudong Department of Chemical Engineering, University of Ottawa, 161 Louis Pasteur, (K1N 6N5), Ottawa, ON, Canada aut Qian Leimin School of Medicine, Jiangsu University, 212013, Zhenjiang, Jiangsu, China aut Duan Lirong School of Medicine, Jiangsu University, 212013, Zhenjiang, Jiangsu, China aut Sun Xianglan School of Medicine, Jiangsu University, 212013, Zhenjiang, Jiangsu, China aut Bu Xuefeng School of Medicine, Jiangsu University, 212013, Zhenjiang, Jiangsu, China aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/9(2010-10-01), 738-745 1071-2690 46:9<738 2010 46 11626 https://doi.org/10.1007/s11626-010-9331-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9331-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Jinbo Department of Orthopedics, The Third Hospital Affiliated to Suzhou University, 213003, Changzhou, China aut NATIONALLICENCE 700 1- Zhang Zhijian School of Medicine, Jiangsu University, 212013, Zhenjiang, Jiangsu, China aut NATIONALLICENCE 700 1- Gong Aihua School of Medicine, Jiangsu University, 212013, Zhenjiang, Jiangsu, China aut NATIONALLICENCE 700 1- Cao Xudong Department of Chemical Engineering, University of Ottawa, 161 Louis Pasteur, (K1N 6N5), Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Qian Leimin School of Medicine, Jiangsu University, 212013, Zhenjiang, Jiangsu, China aut NATIONALLICENCE 700 1- Duan Lirong School of Medicine, Jiangsu University, 212013, Zhenjiang, Jiangsu, China aut NATIONALLICENCE 700 1- Sun Xianglan School of Medicine, Jiangsu University, 212013, Zhenjiang, Jiangsu, China aut NATIONALLICENCE 700 1- Bu Xuefeng School of Medicine, Jiangsu University, 212013, Zhenjiang, Jiangsu, China aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/9(2010-10-01), 738-745 1071-2690 46:9<738 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer