caa a22 4500 445292547 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20100701xx s 000 0 eng 10.1007/s11626-010-9311-5 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9311-5 Effect of daptomycin on primary rat muscle cell cultures in vitro [Elektronische Daten] [Tatiana Kostrominova, Scott Coleman, Frederick Oleson, John Faulkner, Lisa Larkin] Daptomycin is a lipopeptide antibiotic that has strong bactericidal activity against Gram-positive bacteria and that was previously reported to exhibit minor side effects on skeletal muscle. This study was designed to further characterize the effect of daptomycin on skeletal muscle through the use of primary cultures of muscles from rats. Our investigations demonstrated that daptomycin has a concentration-dependent and time-dependent effect on the plasma membrane of primary cultures of differentiated, spontaneously contracting rat myotubes. No effects were evident in non-differentiated myoblasts or other mononucleated cells present in cultures even at the highest daptomycin concentrations tested (6,000µg/mL). In cultures treated with daptomycin at a concentration of 2,000µg/mL, plasma membrane damage was observed in ∼20-30% of differentiated myotubes; no myotube damage was detected at concentrations of 1,000µg/mL and below. A transient loss of spontaneous myotube contractions was evident at 750µg/mL, while at 2,000µg/mL and above, a permanent loss of spontaneous contractility was observed. These results suggest that the putative targets for daptomycin effects on skeletal muscle are structures on the plasma membrane of highly differentiated myotubes. The Society for In Vitro Biology, 2010 Daptomycin nationallicence Plasma membrane nationallicence Myotubes nationallicence Spontaneous contraction nationallicence Membrane damage nationallicence Kostrominova Tatiana Department of Molecular and Integrative Physiology, BSRB, University of Michigan, 48109-2200, Ann Arbor, MI, USA aut Coleman Scott Cubist Pharmaceuticals, 65 Hayden Avenue, 02421, Lexington, MA, USA aut Oleson Frederick Cubist Pharmaceuticals, 65 Hayden Avenue, 02421, Lexington, MA, USA aut Faulkner John Department of Molecular and Integrative Physiology, BSRB, University of Michigan, 48109-2200, Ann Arbor, MI, USA aut Larkin Lisa Department of Molecular and Integrative Physiology, BSRB, University of Michigan, 48109-2200, Ann Arbor, MI, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/7(2010-07-01), 613-618 1071-2690 46:7<613 2010 46 11626 https://doi.org/10.1007/s11626-010-9311-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9311-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kostrominova Tatiana Department of Molecular and Integrative Physiology, BSRB, University of Michigan, 48109-2200, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Coleman Scott Cubist Pharmaceuticals, 65 Hayden Avenue, 02421, Lexington, MA, USA aut NATIONALLICENCE 700 1- Oleson Frederick Cubist Pharmaceuticals, 65 Hayden Avenue, 02421, Lexington, MA, USA aut NATIONALLICENCE 700 1- Faulkner John Department of Molecular and Integrative Physiology, BSRB, University of Michigan, 48109-2200, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Larkin Lisa Department of Molecular and Integrative Physiology, BSRB, University of Michigan, 48109-2200, Ann Arbor, MI, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/7(2010-07-01), 613-618 1071-2690 46:7<613 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer