caa a22 4500 445292563 CHVBK 20180317142532.0 cr unu---uuuuu 170323e20100701xx s 000 0 eng 10.1007/s11626-010-9318-y doi (NATIONALLICENCE)springer-10.1007/s11626-010-9318-y Evaluation of suitable reference gene for real-time PCR in human umbilical cord mesenchymal stem cells with long-term in vitro expansion [Elektronische Daten] [Youwei Wang, Zhibo Han, Shulin Yan, Aibin Mao, Bin Wang, He Ren, Ying Chi, Zhongchao Han] In real-time quantitative PCR, the accuracy of normalized data is highly dependent on the stability of the reference genes. However, reference gene expression in a given cell type or experimental condition can vary considerably. The goal of this study was to establish a reliable set of reference genes for real-time PCR studies using human umbilical cord mesenchymal stem cells with long-term in vitro expansion. The stability of ten potential reference genes was examined in human umbilical cord mesenchymal stem cells. We found that Ywhaz and Rpl13a, not beta-actin or Gapdh, were the most stably expressed of the internal control genes in different passages of human umbilical cord mesenchymal stem cells. Ywhaz and Rpl13a could be used as reference genes for relative gene quantification and normalization purposes in real-time PCR studies of human umbilical cord mesenchymal stem cells. The Society for In Vitro Biology, 2010 Real-time PCR nationallicence geNorm nationallicence Human umbilical cord mesenchymal stem cells nationallicence Reference genes nationallicence Wang Youwei National Engineering Research Center of Cell Products, AmCellGene Co. Ltd, TEDA, 300457, Tianjin, People's Republic of China aut Han Zhibo State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, National Engineering Research Center of Cell Products, 288 Nanjing Road, 300020, Tianjin, People's Republic of China aut Yan Shulin National Engineering Research Center of Cell Products, AmCellGene Co. Ltd, TEDA, 300457, Tianjin, People's Republic of China aut Mao Aibin National Engineering Research Center of Cell Products, AmCellGene Co. Ltd, TEDA, 300457, Tianjin, People's Republic of China aut Wang Bin National Engineering Research Center of Cell Products, AmCellGene Co. Ltd, TEDA, 300457, Tianjin, People's Republic of China aut Ren He National Engineering Research Center of Cell Products, AmCellGene Co. Ltd, TEDA, 300457, Tianjin, People's Republic of China aut Chi Ying State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, National Engineering Research Center of Cell Products, 288 Nanjing Road, 300020, Tianjin, People's Republic of China aut Han Zhongchao National Engineering Research Center of Cell Products, AmCellGene Co. Ltd, TEDA, 300457, Tianjin, People's Republic of China aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/7(2010-07-01), 595-599 1071-2690 46:7<595 2010 46 11626 https://doi.org/10.1007/s11626-010-9318-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9318-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Youwei National Engineering Research Center of Cell Products, AmCellGene Co. Ltd, TEDA, 300457, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Han Zhibo State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, National Engineering Research Center of Cell Products, 288 Nanjing Road, 300020, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Yan Shulin National Engineering Research Center of Cell Products, AmCellGene Co. Ltd, TEDA, 300457, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Mao Aibin National Engineering Research Center of Cell Products, AmCellGene Co. Ltd, TEDA, 300457, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Wang Bin National Engineering Research Center of Cell Products, AmCellGene Co. Ltd, TEDA, 300457, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Ren He National Engineering Research Center of Cell Products, AmCellGene Co. Ltd, TEDA, 300457, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Chi Ying State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, National Engineering Research Center of Cell Products, 288 Nanjing Road, 300020, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Han Zhongchao National Engineering Research Center of Cell Products, AmCellGene Co. Ltd, TEDA, 300457, Tianjin, People's Republic of China aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/7(2010-07-01), 595-599 1071-2690 46:7<595 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer