caa a22 4500 445292571 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100701xx s 000 0 eng 10.1007/s11626-010-9315-1 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9315-1 Tissue culture: the unlimited potential [Elektronische Daten] [Gordon Sato, J. Sato, Tetsuji Okamoto, Wallace McKeehan, David Barnes] Lack of tissue-specific differentiated functions of cells in tissue culture, once thought to be due to "dedifferentiation”, was shown to be due to selective overgrowth of fibroblasts by a series of simple experiments that challenged the prevailing dogma. Following this insight, enrichment culture techniques (alternate animal and culture passage) were designed to give functionally differentiated tumor cells selective advantage over the fibroblasts. These experiments resulted in the derivation of a large number of functionally differentiated clonal strains of a range of cell types, providing the final point of destruction of the dogma of "dedifferentiation.” Instead, the hypothesis was proposed that cells in culture accurately represent cells in vivo, but without the complex in vivo environment. With the development of hormonally defined media and its combination with functionally differentiated clonal cell lines, this concept has been strengthened and the potential of tissue culture studies has been greatly augmented. Hormonally defined media allow the culture of cell types that cannot be grown in conventional, serum-supplemented media. These approaches demonstrate that hormonal responses and dependencies can be discovered in culture. Following this thinking and the discovery of hormonal dependencies of cancer cells has led to a new rationale for therapy. Tissue culture and cell technology continue to play an important role in solving human health problems. The Society for In Vitro Biology, 2010 Alternate animal and culture passage nationallicence Enrichment culture nationallicence Dedifferentiation nationallicence Hormonally defined nationallicence Selective overgrowth nationallicence Sato Gordon The Manzanar Project Foundation, 27 Cedar St., 01984, Wenham, MA, USA aut Sato J. The Manzanar Project Foundation, 27 Cedar St., 01984, Wenham, MA, USA aut Okamoto Tetsuji The Graduate School of Biomedical Sciences, Hiroshima University, 734-8551, Hiroshima, Japan aut McKeehan Wallace Institute of Biosciences and Technology, Texas A&M University System Health Science Center, 2121 Holcombe Blvd., 77030, Houston, TX, USA aut Barnes David Mount Desert Island Biological Laboratory, Old Bar Harbor Rd., 04672, Salisbury Cove, ME, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/7(2010-07-01), 590-594 1071-2690 46:7<590 2010 46 11626 https://doi.org/10.1007/s11626-010-9315-1 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9315-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sato Gordon The Manzanar Project Foundation, 27 Cedar St., 01984, Wenham, MA, USA aut NATIONALLICENCE 700 1- Sato J. The Manzanar Project Foundation, 27 Cedar St., 01984, Wenham, MA, USA aut NATIONALLICENCE 700 1- Okamoto Tetsuji The Graduate School of Biomedical Sciences, Hiroshima University, 734-8551, Hiroshima, Japan aut NATIONALLICENCE 700 1- McKeehan Wallace Institute of Biosciences and Technology, Texas A&M University System Health Science Center, 2121 Holcombe Blvd., 77030, Houston, TX, USA aut NATIONALLICENCE 700 1- Barnes David Mount Desert Island Biological Laboratory, Old Bar Harbor Rd., 04672, Salisbury Cove, ME, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/7(2010-07-01), 590-594 1071-2690 46:7<590 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer