caa a22 4500 44529258X CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100701xx s 000 0 eng 10.1007/s11626-010-9314-2 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9314-2 Differentiation of cryopreserved human umbilical cord blood-derived stromal cells into cells with an oligodendrocyte phenotype [Elektronische Daten] [Yong-Chun Luo, Hong-Tian Zhang, Hao-Yu Cheng, Zhi-Jun Yang, Yi-Wu Dai, Ru-Xiang Xu] In this study, we examined the phenotypic characteristics of human umbilical cord blood-derived mesenchymal stromal cells (UCB-derived MSCs) differentiated along an oligodendrocyte pathway. We induced human UCB-derived MSCs to form floating neurospheres, and these neurospheres were then induced to differentiate into oligodendrocyte progenitor-like cells using multiple induction factors. Differentiated UCB-derived MSCs showed morphologic characteristics of an oligodendrocyte phenotype. The expression of cell surface markers characteristic of oligodendrocyte progenitor cells or oligodendrocytes was determined by immunocytochemical staining. These results suggest that human UCB-derived MSCs can be induced to differentiate into cells with an oligodendrocyte phenotype and that these cells may have potential in the future cellular therapy of central neurological disorders. The Society for In Vitro Biology, 2010 Umbilical cord blood nationallicence Mesenchymal stromal cells nationallicence Oligodendrocyte nationallicence Transdifferentiation nationallicence Luo Yong-Chun Department of Neurosurgery, Military General Hospital of Beijing PLA, 100700, Beijing, China aut Zhang Hong-Tian Department of Neurosurgery, Military General Hospital of Beijing PLA, 100700, Beijing, China aut Cheng Hao-Yu Department of Hematology, Tumor Hospital of Yunnan Province, 650118, Kunming, China aut Yang Zhi-Jun Department of Neurosurgery, Military General Hospital of Beijing PLA, 100700, Beijing, China aut Dai Yi-Wu Department of Neurosurgery, Military General Hospital of Beijing PLA, 100700, Beijing, China aut Xu Ru-Xiang Department of Neurosurgery, Military General Hospital of Beijing PLA, 100700, Beijing, China aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/7(2010-07-01), 585-589 1071-2690 46:7<585 2010 46 11626 https://doi.org/10.1007/s11626-010-9314-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9314-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luo Yong-Chun Department of Neurosurgery, Military General Hospital of Beijing PLA, 100700, Beijing, China aut NATIONALLICENCE 700 1- Zhang Hong-Tian Department of Neurosurgery, Military General Hospital of Beijing PLA, 100700, Beijing, China aut NATIONALLICENCE 700 1- Cheng Hao-Yu Department of Hematology, Tumor Hospital of Yunnan Province, 650118, Kunming, China aut NATIONALLICENCE 700 1- Yang Zhi-Jun Department of Neurosurgery, Military General Hospital of Beijing PLA, 100700, Beijing, China aut NATIONALLICENCE 700 1- Dai Yi-Wu Department of Neurosurgery, Military General Hospital of Beijing PLA, 100700, Beijing, China aut NATIONALLICENCE 700 1- Xu Ru-Xiang Department of Neurosurgery, Military General Hospital of Beijing PLA, 100700, Beijing, China aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/7(2010-07-01), 585-589 1071-2690 46:7<585 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer