caa a22 4500 445292598 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100701xx s 000 0 eng 10.1007/s11626-010-9312-4 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9312-4 Expression of stemness markers in mouse parthenogenetic-diploid blastocysts is influenced by slight variation of activation protocol adopted [Elektronische Daten] [Enrica Bianchi, Raffaele Geremia, Claudio Sette] The importance of obtaining stem cells through alternative methods has increased progressively in the recent years due to the potential role that embryonic stem (ES) cells play in the field of regenerative medicine. In this regard, generation of parthenogenetic blastocysts allows the production of ethic-free ES cells without the need to manipulate normal embryos. Our work was aimed at clarifying whether variations in the method adopted to generate diploid parthenogenetic blastocysts could determine differences in the quality of blastocysts produced. In vitro development of mouse oocytes activated with three protocols, using Sr2+ and cytochalasin for different time, was compared with that of in vivo fertilized embryos. We have evaluated the efficiency of blastocyst formation and analysed the expression pattern of the stemness markers OCT4, CDX2, and NANOG. Our results indicate that the yield of diploid parthenogenotes and the segregation of the stemness marker OCT4 in the developing blastocyst are influenced by the parthenogenetic protocol adopted. Particularly, even if all methods tested allowed the production of blastocysts in vitro, the correct segregation of OCT4 occurred only in blastocysts developed from oocytes concomitantly treated for 4h with Sr2+ and cytochalasin D. Our results indicate that the protocol employed to develop parthenogenetic blastocysts in vitro affects the quality of cells in the inner cell mass. The Society for In Vitro Biology, 2010 Blastocysts nationallicence Cytochalasin D nationallicence Oct4 nationallicence Parthenogenesis nationallicence Sr2+ nationallicence Bianchi Enrica Department of Public Health and Cell Biology, Section of Anatomy, University of Rome "Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy aut Geremia Raffaele Department of Public Health and Cell Biology, Section of Anatomy, University of Rome "Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy aut Sette Claudio Department of Public Health and Cell Biology, Section of Anatomy, University of Rome "Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/7(2010-07-01), 619-623 1071-2690 46:7<619 2010 46 11626 https://doi.org/10.1007/s11626-010-9312-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9312-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bianchi Enrica Department of Public Health and Cell Biology, Section of Anatomy, University of Rome "Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy aut NATIONALLICENCE 700 1- Geremia Raffaele Department of Public Health and Cell Biology, Section of Anatomy, University of Rome "Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy aut NATIONALLICENCE 700 1- Sette Claudio Department of Public Health and Cell Biology, Section of Anatomy, University of Rome "Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/7(2010-07-01), 619-623 1071-2690 46:7<619 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer