caa a22 4500 445292628 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100701xx s 000 0 eng 10.1007/s11626-010-9316-0 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9316-0 Ovine bone marrow mesenchymal stem cells: isolation and characterization of the cells and their osteogenic differentiation potential on embroidered and surface-modified polycaprolactone-co-lactide scaffolds [Elektronische Daten] [C. Rentsch, R. Hess, B. Rentsch, A. Hofmann, S. Manthey, D. Scharnweber, A. Biewener, H. Zwipp] The current study was undertaken with the goal being isolation, cultivation, and characterization of ovine mesenchymal stem cells (oMSC). Furthermore, the objective was to determine whether biological active polycaprolactone-co-lactide (trade name PCL) scaffolds support the growth and differentiation of oMSC in vitro. The oMSC were isolated from the iliac crest of six merino sheep. Three factors were used to demonstrate the MSC properties of the isolated cells in detail. (1) Their ability to proliferate in culture with a spindle-shaped morphology, (2) presence of specific surface marker proteins, and (3) their capacity to differentiate into the three classical mesenchymal pathways, osteoblastic, adipogenic, and chondrogenic lineages. Furthermore, embroidered PCL scaffolds were coated with collagen I (coll I) and chondroitin sulfate (CS). The porous structure of the scaffolds and the coating with coll I/CS allowed the oMSC to adhere, proliferate, and to migrate into the scaffolds. The collI/CS coating on the PCL scaffolds induced osteogenic differentiation of hMSC, without differentiation supplements, indicating that the scaffold also has an osteoinductive character. In conclusion, the isolated cells from the ovine bone marrow have similar morphologic, immunophenotypic, and functional characteristics as their human counterparts. These cells were also found to differentiate into multiple mesenchymal cell types. This study demonstrates that embroidered PCL scaffolds can act as a temporary matrix for cell migration, proliferation, and differentiation of oMSC. The data presented will provide a reliable model system to assess the translation of MSC-based therapy into a variety of valuable ovine experimental models under autologous settings. The Society for In Vitro Biology, 2010 Ovine mesenchymal stem cells nationallicence Bone marrow nationallicence Multilineage differentiation nationallicence Polycaprolactone-co-lactide scaffold nationallicence Tissue engineering nationallicence Rentsch C. Department of Trauma and Reconstructive Surgery, University Hospital Carl Gustav Carus Dresden, Fetscher Straße 74, 01307, Dresden, Germany aut Hess R. Max Bergmann Center of Biomaterials, Budapester Straße 27, 01069, Dresden, Germany aut Rentsch B. Catgut GmbH, Gewerbepark 18, 08258, Markneukirchen, Germany aut Hofmann A. Max Bergmann Center of Biomaterials, Budapester Straße 27, 01069, Dresden, Germany aut Manthey S. Department of Trauma and Reconstructive Surgery, University Hospital Carl Gustav Carus Dresden, Fetscher Straße 74, 01307, Dresden, Germany aut Scharnweber D. Max Bergmann Center of Biomaterials, Budapester Straße 27, 01069, Dresden, Germany aut Biewener A. Department of Trauma and Reconstructive Surgery, University Hospital Carl Gustav Carus Dresden, Fetscher Straße 74, 01307, Dresden, Germany aut Zwipp H. Department of Trauma and Reconstructive Surgery, University Hospital Carl Gustav Carus Dresden, Fetscher Straße 74, 01307, Dresden, Germany aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/7(2010-07-01), 624-634 1071-2690 46:7<624 2010 46 11626 https://doi.org/10.1007/s11626-010-9316-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9316-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rentsch C. Department of Trauma and Reconstructive Surgery, University Hospital Carl Gustav Carus Dresden, Fetscher Straße 74, 01307, Dresden, Germany aut NATIONALLICENCE 700 1- Hess R. Max Bergmann Center of Biomaterials, Budapester Straße 27, 01069, Dresden, Germany aut NATIONALLICENCE 700 1- Rentsch B. Catgut GmbH, Gewerbepark 18, 08258, Markneukirchen, Germany aut NATIONALLICENCE 700 1- Hofmann A. Max Bergmann Center of Biomaterials, Budapester Straße 27, 01069, Dresden, Germany aut NATIONALLICENCE 700 1- Manthey S. Department of Trauma and Reconstructive Surgery, University Hospital Carl Gustav Carus Dresden, Fetscher Straße 74, 01307, Dresden, Germany aut NATIONALLICENCE 700 1- Scharnweber D. Max Bergmann Center of Biomaterials, Budapester Straße 27, 01069, Dresden, Germany aut NATIONALLICENCE 700 1- Biewener A. Department of Trauma and Reconstructive Surgery, University Hospital Carl Gustav Carus Dresden, Fetscher Straße 74, 01307, Dresden, Germany aut NATIONALLICENCE 700 1- Zwipp H. Department of Trauma and Reconstructive Surgery, University Hospital Carl Gustav Carus Dresden, Fetscher Straße 74, 01307, Dresden, Germany aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/7(2010-07-01), 624-634 1071-2690 46:7<624 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer