caa a22 4500 445292636 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100701xx s 000 0 eng 10.1007/s11626-010-9310-6 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9310-6 Phenotypic and physiologic variability in nasal epithelium cultured from smokers and non-smokers exposed to secondhand tobacco smoke [Elektronische Daten] [Johnny Carson, Tsui-Shan Lu, Luisa Brighton, Milan Hazucha, Ilona Jaspers, Haibo Zhou] The emergence of air-liquid interface (ALI) culturing of mammalian airway epithelium is a recent innovation for experimental modeling of airway epithelial development, function, and pathogenic mechanisms associated with infectious agent and irritant exposure. This construct provides an experimental platform for in vitro propagation, manipulation, and testing of airway epithelium in a structural and physiologic state that emulates in vivo organization. In this study, we have cultured nasal epithelial biopsies from human subjects with variable histories of tobacco smoke exposure and assessed ciliary beat frequency (CBF) after an extended interval in vitro relative to CBF determined on biopsies from the same subjects immediately upon acquisition. We observed elevated CBF in nasal epithelial biopsies as well as persistence of accelerated CBF in ALI cultures deriving from biopsies of smokers and non-smokers exposed to environmental tobacco smoke compared to CBF in cultures from biopsies of well-documented non-smokers. Moreover, cultures deriving from smokers exhibited reduced ciliation as the cultures matured. These studies document that nasal epithelium cultured in the ALI system retains physiologic and phenotypic characteristics of the epithelial layer in vivo even through rounds of proliferative expansion. These observations suggest that stable epigenetic factors affecting regulation of ciliary function and phenotype commitment may be operative. The Society for In Vitro Biology, 2010 Cilia nationallicence Epithelium nationallicence Tobacco smoke nationallicence Inhalation toxicology nationallicence Cell culture nationallicence Carson Johnny Department of Pediatrics, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA aut Lu Tsui-Shan The Department of Mathematics, National Taiwan Normal University, Taipei, Taiwan, Republic of China aut Brighton Luisa Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, CB #7310, 104 Mason Farm Road, 27599-7310, Chapel Hill, NC, USA aut Hazucha Milan Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, CB #7310, 104 Mason Farm Road, 27599-7310, Chapel Hill, NC, USA aut Jaspers Ilona Department of Pediatrics, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA aut Zhou Haibo Department of Biostatistics, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/7(2010-07-01), 606-612 1071-2690 46:7<606 2010 46 11626 https://doi.org/10.1007/s11626-010-9310-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9310-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carson Johnny Department of Pediatrics, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA aut NATIONALLICENCE 700 1- Lu Tsui-Shan The Department of Mathematics, National Taiwan Normal University, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Brighton Luisa Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, CB #7310, 104 Mason Farm Road, 27599-7310, Chapel Hill, NC, USA aut NATIONALLICENCE 700 1- Hazucha Milan Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, CB #7310, 104 Mason Farm Road, 27599-7310, Chapel Hill, NC, USA aut NATIONALLICENCE 700 1- Jaspers Ilona Department of Pediatrics, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA aut NATIONALLICENCE 700 1- Zhou Haibo Department of Biostatistics, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/7(2010-07-01), 606-612 1071-2690 46:7<606 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer