caa a22 4500 445292644 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100701xx s 000 0 eng 10.1007/s11626-010-9328-9 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9328-9 Preparation and physical properties of a novel biocompatible porcine corneal acellularized matrix [Elektronische Daten] [Yi Shao, Luowa Quyang, Yueping Zhou, Jing Tang, Yuehui Tan, Qiuping Liu, Zhirong Lin, Tingting Yin, Fangfang Qiu, Zuguo Liu] This study was to investigate the stability, physico-mechanical property and biocompatibility of porcine corneal acellularized matrix (PCAM) that was prepared using human sera treatment to decellularize corneas. The stability (the rate of biodegradation) and physico-mechanical property (water uptake, density, and porosity) of PCAM were not compromised, compared with porcine fresh cornea matrix (PFCM, p > 0.05). The contact and extract cytotoxicity tests with human corneal epithelial cells and human keratocytes showed that PCAM has a good biocompatibility ex vivo and no cytotoxic effect. These results present the ability to create safety scaffolds that function as cornea grafts and provide a novel experimental approach for the study of cornea tissue engineering using acellular porcine cornea. The Society for In Vitro Biology, 2010 Cornea matrix nationallicence Human sera nationallicence Tissue engineering nationallicence Decellularization biocompatibily nationallicence Shao Yi Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology of Sun Yat-sen University, Guangzhou, Guangdong Province, China aut Quyang Luowa Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China aut Zhou Yueping Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China aut Tang Jing Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China aut Tan Yuehui Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China aut Liu Qiuping Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China aut Lin Zhirong Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology of Sun Yat-sen University, Guangzhou, Guangdong Province, China aut Yin Tingting Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China aut Qiu Fangfang Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China aut Liu Zuguo Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/7(2010-07-01), 600-605 1071-2690 46:7<600 2010 46 11626 https://doi.org/10.1007/s11626-010-9328-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9328-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shao Yi Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology of Sun Yat-sen University, Guangzhou, Guangdong Province, China aut NATIONALLICENCE 700 1- Quyang Luowa Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China aut NATIONALLICENCE 700 1- Zhou Yueping Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China aut NATIONALLICENCE 700 1- Tang Jing Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China aut NATIONALLICENCE 700 1- Tan Yuehui Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China aut NATIONALLICENCE 700 1- Liu Qiuping Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China aut NATIONALLICENCE 700 1- Lin Zhirong Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology of Sun Yat-sen University, Guangzhou, Guangdong Province, China aut NATIONALLICENCE 700 1- Yin Tingting Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China aut NATIONALLICENCE 700 1- Qiu Fangfang Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China aut NATIONALLICENCE 700 1- Liu Zuguo Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/7(2010-07-01), 600-605 1071-2690 46:7<600 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer