caa a22 4500 445292652 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20101201xx s 000 0 eng 10.1007/s11626-010-9351-x doi (NATIONALLICENCE)springer-10.1007/s11626-010-9351-x The effect of physiological concentrations of sex hormones, insulin, and glucagon on growth of breast and prostate cells supplemented with unmodified human serum [Elektronische Daten] [Amin Esfahani, Cyril Kendall, Balachandran Bashyam, Michael Archer, David Jenkins] The majority of cell culture studies have assessed the effect of hormones on cancer cell growth using media supplemented with charcoal-treated fetal bovine serum (CTS). We aimed to determine whether using a system more reflective of the human condition by changing the charcoal-treated serum to an untreated pooled human serum (PHS) resulted in the same hormone responses in breast and prostate cell lines. MCF-7 breast cancer, MCF-10A non-transformed breast, and LNCaP prostate cancer cell lines supplemented with PHS were treated with high and low physiological concentrations of six hormones (17β-estradiol, dehydroepiandosterone (DHEA), dihydrotestosterone (DHT), testosterone, insulin, and glucagon). Cell growth was measured after 72h of incubation. All hormones stimulated growth of MCF-7 cells (p < 0.05). MCF-10A cell growth was inhibited by DHEA, DHT, and testosterone (p < 0.05), unaffected by 17β-estradiol and glucagon, and stimulated by insulin (p < 0.05). LNCaP cell growth was stimulated by the highest concentration of DHEA and DHT (p < 0.05) and inhibited by the highest concentration of 17β-estradiol (p < 0.05), while insulin and testosterone, had no effect. Overall, PHS lowered the magnitude of the effect of hormones on cell growth in comparison to CTS. Due to the presence of all serum constituents, our model represents a more appropriate physiological environment for determining the effect of hormones on cancer cell growth. Further studies are required to determine the mechanisms by which added hormones interact with the constituents of untreated human serum. The Society for In Vitro Biology, 2010 Breast cancer nationallicence Prostate cancer nationallicence Sex hormones nationallicence Glucagon nationallicence Insulin nationallicence Esfahani Amin Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, M5S 3E2, Toronto, Ontario, Canada aut Kendall Cyril Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, M5S 3E2, Toronto, Ontario, Canada aut Bashyam Balachandran Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, M5S 3E2, Toronto, Ontario, Canada aut Archer Michael Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, M5S 3E2, Toronto, Ontario, Canada aut Jenkins David Department of Medicine, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/10(2010-12-01), 856-862 1071-2690 46:10<856 2010 46 11626 https://doi.org/10.1007/s11626-010-9351-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9351-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Esfahani Amin Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, M5S 3E2, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Kendall Cyril Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, M5S 3E2, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Bashyam Balachandran Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, M5S 3E2, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Archer Michael Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, M5S 3E2, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Jenkins David Department of Medicine, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/10(2010-12-01), 856-862 1071-2690 46:10<856 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer