caa a22 4500 445292679 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20101201xx s 000 0 eng 10.1007/s11626-010-9361-8 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9361-8 Gamma-irradiation depletes endogenous germ cells and increases donor cell distribution in chimeric chickens [Elektronische Daten] [Kyung Park, Seok Kang, Tae Kim, Young Lee, Hyung Lee, Gwonhwa Song, Jae Han] The production of chimeric birds is an important tool for the investigation of vertebrate development, the conservation of endangered birds, and the development of various biotechnological applications. This study examined whether gamma (γ)-irradiation depletes endogenous primordial germ cells and enhances the efficiency of somatic chimerism in chickens. An optimal irradiation protocol for stage X embryos was determined after irradiation at various doses (0, 100, 300, 500, 600, 700, and 2,000rad). Exposure to 500rad of γ-irradiation for 73s significantly decreased the number of primordial germ cells (P < 0.0001). Somatic chimera hatchlings were then produced by transferring blastodermal cells from a Korean Oge into either an irradiated (at 500rad) or intact stage X White Leghorn embryo. An analysis of feather color pattern and polymerase chain reaction-based species-specific amplification of various tissues of the hatchlings confirmed chimerism in most organs of the chick produced from the irradiated recipient; a lesser degree of chimerism was observed in the non-irradiated control recipient. In conclusion, the exposure of chick embryos to an optimized dose of γ-irradiation effectively depleted germ cells and yielded greater somatic chimerism than non-irradiated control embryos. This technique can be applied to interspecies reproduction or the production of transgenic birds. The Society for In Vitro Biology, 2010 Chimera nationallicence Gamma (γ)-irradiation nationallicence Primordial germ cells (PGCs) nationallicence Stage X embryos nationallicence Park Kyung WCU Biomodulation Major, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut Kang Seok WCU Biomodulation Major, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut Kim Tae WCU Biomodulation Major, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut Lee Young WCU Biomodulation Major, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut Lee Hyung WCU Biomodulation Major, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut Song Gwonhwa WCU Biomodulation Major, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut Han Jae WCU Biomodulation Major, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/10(2010-12-01), 828-833 1071-2690 46:10<828 2010 46 11626 https://doi.org/10.1007/s11626-010-9361-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9361-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Park Kyung WCU Biomodulation Major, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 700 1- Kang Seok WCU Biomodulation Major, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Tae WCU Biomodulation Major, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Young WCU Biomodulation Major, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Hyung WCU Biomodulation Major, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 700 1- Song Gwonhwa WCU Biomodulation Major, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 700 1- Han Jae WCU Biomodulation Major, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/10(2010-12-01), 828-833 1071-2690 46:10<828 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer