caa a22 4500 445292695 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20101201xx s 000 0 eng 10.1007/s11626-010-9349-4 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9349-4 Development and characterization of new cell line BPH22 from midgut epithelial cells of Poekilocerus pictus (Fabricius, 1775) [Elektronische Daten] [Kiran Kharat, Madhavi Sawant, Shiney Peter, Bhagyashree Hardikar] Poekilocerus pictus is a painted grasshopper, which feeds on Calotropis sp. containing the cardiac glycosides. A new cell line BPH22 is developed from midgut of P. pictus to study its unique physiology and biochemistry. Initially, the Graces insect medium is used with 10% (v/v) fetal bovine serum. After four passages, the serum quantity was reduced up to 0%.The lag phase in growth curve was 5d and log phase is up to 10-11d. The addition of Calotropis extract in Graces medium enhanced the growth of cells. The Calotropis extract with Graces medium altered the morphology of cells of BPH22. The amplification and sequencing of 16S rRNA gene confirmed the origin and purity of the BPH22. The Society for In Vitro Biology, 2010 Poekilocerus pictus nationallicence Midgut cell line nationallicence BPH22 nationallicence Kharat Kiran Department of Zoology, Ket's V. G. Vaze College, Mulund (E), 400081, Mumbai, India aut Sawant Madhavi Department of Zoology, Ket's V. G. Vaze College, Mulund (E), 400081, Mumbai, India aut Peter Shiney Department of Zoology, Ket's V. G. Vaze College, Mulund (E), 400081, Mumbai, India aut Hardikar Bhagyashree Department of Zoology, Ket's V. G. Vaze College, Mulund (E), 400081, Mumbai, India aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/10(2010-12-01), 824-827 1071-2690 46:10<824 2010 46 11626 https://doi.org/10.1007/s11626-010-9349-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9349-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kharat Kiran Department of Zoology, Ket's V. G. Vaze College, Mulund (E), 400081, Mumbai, India aut NATIONALLICENCE 700 1- Sawant Madhavi Department of Zoology, Ket's V. G. Vaze College, Mulund (E), 400081, Mumbai, India aut NATIONALLICENCE 700 1- Peter Shiney Department of Zoology, Ket's V. G. Vaze College, Mulund (E), 400081, Mumbai, India aut NATIONALLICENCE 700 1- Hardikar Bhagyashree Department of Zoology, Ket's V. G. Vaze College, Mulund (E), 400081, Mumbai, India aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/10(2010-12-01), 824-827 1071-2690 46:10<824 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer