caa a22 4500 445292717 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20101201xx s 000 0 eng 10.1007/s11626-010-9352-9 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9352-9 Short tandem repeat profiling: part of an overall strategy for reducing the frequency of cell misidentification [Elektronische Daten] [Raymond Nims, Greg Sykes, Karin Cottrill, Pranvera Ikonomi, Eugene Elmore] The role of cell authentication in biomedical science has received considerable attention, especially within the past decade. This quality control attribute is now beginning to be given the emphasis it deserves by granting agencies and by scientific journals. Short tandem repeat (STR) profiling, one of a few DNA profiling technologies now available, is being proposed for routine identification (authentication) of human cell lines, stem cells, and tissues. The advantage of this technique over methods such as isoenzyme analysis, karyotyping, human leukocyte antigen typing, etc., is that STR profiling can establish identity to the individual level, provided that the appropriate number and types of loci are evaluated. To best employ this technology, a standardized protocol and a data-driven, quality-controlled, and publically searchable database will be necessary. This public STR database (currently under development) will enable investigators to rapidly authenticate human-based cultures to the individual from whom the cells were sourced. Use of similar approaches for non-human animal cells will require developing other suitable loci sets. While implementing STR analysis on a more routine basis should significantly reduce the frequency of cell misidentification, additional technologies may be needed as part of an overall authentication paradigm. For instance, isoenzyme analysis, PCR-based DNA amplification, and sequence-based barcoding methods enable rapid confirmation of a cell line's species of origin while screening against cross-contaminations, especially when the cells present are not recognized by the species-specific STR method. Karyotyping may also be needed as a supporting tool during establishment of an STR database. Finally, good cell culture practices must always remain a major component of any effort to reduce the frequency of cell misidentification. The Author(s), 2010 Cell line authentication nationallicence STR profiling nationallicence Isoenzyme analysis nationallicence Karyotyping nationallicence DNA barcoding nationallicence Nims Raymond RMC Pharmaceutical Solutions, Inc, 2150 Miller Drive, Suite A, 80501, Longmont, CO, USA aut Sykes Greg ATCC, 10801 University Blvd., 20110, Manassas, VA, USA aut Cottrill Karin ATCC, 10801 University Blvd., 20110, Manassas, VA, USA aut Ikonomi Pranvera ATCC, 10801 University Blvd., 20110, Manassas, VA, USA aut Elmore Eugene Department of Radiation Oncology, University of California, Medical Sciences I, B146D, 92697, Irvine, CA, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/10(2010-12-01), 811-819 1071-2690 46:10<811 2010 46 11626 https://doi.org/10.1007/s11626-010-9352-9 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9352-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nims Raymond RMC Pharmaceutical Solutions, Inc, 2150 Miller Drive, Suite A, 80501, Longmont, CO, USA aut NATIONALLICENCE 700 1- Sykes Greg ATCC, 10801 University Blvd., 20110, Manassas, VA, USA aut NATIONALLICENCE 700 1- Cottrill Karin ATCC, 10801 University Blvd., 20110, Manassas, VA, USA aut NATIONALLICENCE 700 1- Ikonomi Pranvera ATCC, 10801 University Blvd., 20110, Manassas, VA, USA aut NATIONALLICENCE 700 1- Elmore Eugene Department of Radiation Oncology, University of California, Medical Sciences I, B146D, 92697, Irvine, CA, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/10(2010-12-01), 811-819 1071-2690 46:10<811 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer