caa a22 4500 445292776 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100901xx s 000 0 eng 10.1007/s11626-010-9322-2 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9322-2 Suppression of embryonic lung branching morphogenesis by antisense oligonucleotides against HOM/C homeobox factors [Elektronische Daten] [Tatsuya Yoshimi, Fumiko Hashimoto, Shigeru Takahashi, Yuji Takahashi] The role of HOM/C homeobox genes on rat embryonic lung branching morphogenesis was investigated using the lung bud explant culture system in an air/liquid interface. Knock down of homeobox b3 and b4 expression by antisense oligonucleotide treatment repressed airway branch formation, while antisense oligonucleotide against homeobox a3 showed no effect. Addition of antisense Hoxb3 oligonucleotide resulted in upregulation of collagen type III mRNA and fibroblast growth factor 10mRNA, while that of the T-box regulatory factor-4 was decreased. Consequently, expression of Clara cell-specific secretory protein was decreased. These results suggest a critical role for homeobox b3 and b4 genes in lung airway branching morphogenesis. The Society for In Vitro Biology, 2010 Lung nationallicence Branching morphogenesis nationallicence Homeobox nationallicence Collagen nationallicence FGF10 nationallicence Yoshimi Tatsuya Laboratory of Environmental Molecular Physiology, School of Life Sciences, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, 192-0392, Hachioji, Tokyo, Japan aut Hashimoto Fumiko Laboratory of Environmental Molecular Physiology, School of Life Sciences, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, 192-0392, Hachioji, Tokyo, Japan aut Takahashi Shigeru Laboratory of Environmental Molecular Physiology, School of Life Sciences, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, 192-0392, Hachioji, Tokyo, Japan aut Takahashi Yuji Laboratory of Environmental Molecular Physiology, School of Life Sciences, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, 192-0392, Hachioji, Tokyo, Japan aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/8(2010-09-01), 664-672 1071-2690 46:8<664 2010 46 11626 https://doi.org/10.1007/s11626-010-9322-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9322-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yoshimi Tatsuya Laboratory of Environmental Molecular Physiology, School of Life Sciences, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, 192-0392, Hachioji, Tokyo, Japan aut NATIONALLICENCE 700 1- Hashimoto Fumiko Laboratory of Environmental Molecular Physiology, School of Life Sciences, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, 192-0392, Hachioji, Tokyo, Japan aut NATIONALLICENCE 700 1- Takahashi Shigeru Laboratory of Environmental Molecular Physiology, School of Life Sciences, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, 192-0392, Hachioji, Tokyo, Japan aut NATIONALLICENCE 700 1- Takahashi Yuji Laboratory of Environmental Molecular Physiology, School of Life Sciences, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, 192-0392, Hachioji, Tokyo, Japan aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/8(2010-09-01), 664-672 1071-2690 46:8<664 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer