caa a22 4500 445292784 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100901xx s 000 0 eng 10.1007/s11626-010-9325-z doi (NATIONALLICENCE)springer-10.1007/s11626-010-9325-z Culture of newborn monkey liver epithelial progenitor cells in chemical defined serum-free medium [Elektronische Daten] [Shaohui Ji, Lifang Jin, Xiangyu Guo, Weizhi Ji] Studies with hepatic progenitor cells from non-human primates would allow better understanding of their human counterparts. In this study, rhesus monkey liver epithelial progenitor cells (mLEPCs) were derived from a small piece of newborn livers in chemical defined serum-free medium. Digested hepatic cells were treated in Ca2+-containing medium to form cell aggregates. Two types of cell aggregates were generated: elongated spindle cells and polygonal epithelial cells. Elongated spindle cells were expressed as vimentin and brachyury, and they were disappeared within 5d in our cultures. The remaining type consisted of small polygonal epithelial cells that expressed cytokeratin 7 (CK7), CK8, CK18, nestin, CD49f, and E-cad, the markers of hepatic stem cells, but were negative for α-fetoprotein, albumin, and CK19. They can proliferate and be passaged, if on laminin or rat tail collagen gel, to initiate colonies. When cultured with dexamethasone and oncostatin M, the expression of mature hepatocyte markers, such as α-1-antitrypsin, intracytoplasmic glycogen storage, indocyanine green uptake, and lipid droplet generation, were induced in differentiated cells. If transferred onto mouse embryonic fibroblasts feeders, they gave rise to CK19-positive cholangiocytes with formation of doughnut-like structure. Thus, mLEPCs with bipotency were derived from newborn monkey liver and may serve as a preclinical model for assessment of cell therapy in humans. The Society for In Vitro Biology, 2010 Hepatic progenitor cells nationallicence Bipotency nationallicence Serum free nationallicence Extracelullar matrix nationallicence Rhesus monkey nationallicence Ji Shaohui Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, 32# Jiaochang Donglu, 650223, Kunming, Yunnan, China aut Jin Lifang Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, 32# Jiaochang Donglu, 650223, Kunming, Yunnan, China aut Guo Xiangyu Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, 32# Jiaochang Donglu, 650223, Kunming, Yunnan, China aut Ji Weizhi Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, 32# Jiaochang Donglu, 650223, Kunming, Yunnan, China aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/8(2010-09-01), 693-701 1071-2690 46:8<693 2010 46 11626 https://doi.org/10.1007/s11626-010-9325-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9325-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ji Shaohui Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, 32# Jiaochang Donglu, 650223, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Jin Lifang Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, 32# Jiaochang Donglu, 650223, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Guo Xiangyu Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, 32# Jiaochang Donglu, 650223, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Ji Weizhi Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, 32# Jiaochang Donglu, 650223, Kunming, Yunnan, China aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/8(2010-09-01), 693-701 1071-2690 46:8<693 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer