caa a22 4500 445292792 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100901xx s 000 0 eng 10.1007/s11626-010-9321-3 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9321-3 SREBP isoform and SREBP target gene expression during rat primary hepatocyte culture [Elektronische Daten] [Jiakai Wu, Alan Dickson] Expression of mRNA encoding sterol regulatory element binding protein (SREBP) isoforms (SREBP-1a, -1c, -2) and seven SREBP target genes decreased dramatically as a result of isolation and subsequent culture of primary rat hepatocytes. In standard maintenance medium (MM) expression remained low but when cultured in HepatoZYME (HZM), there was a selective increase in mRNA encoding SREBP-2 and a subset of SREBP target genes, a group characterised by promoters containing adjacent sterol regulatory element and nuclear factor Y (NF-Y) binding sequences. Quantification of all three NF-Y transcripts showed that expression of nuclear factor Y α subunit and nuclear factor Y β subunit mRNA increased during culture in HZM (in contrast to the situation with MM) whilst specificity protein 1, liver-x-receptor and hepatocyte nuclear factor-4α mRNA exhibited equivalent decreased expression in both HZM and MM. Our data indicate that HZM exerts a selective preservation of hepatocyte phenotype through actions on NF-Y expression directly or via an effect secondary to actions on SREBP-2 expression. These data add to the molecular dissection of the causes of hepatocyte dedifferentiation during culture and address means to develop approaches to prevent/limit phenotype change. The Society for In Vitro Biology, 2010 NF-Y nationallicence LXR nationallicence Sp1 nationallicence HMGCoA reductase nationallicence HepatoZYME nationallicence Wu Jiakai Faculty of Life Sciences, The University of Manchester, The Michael Smith Building, Oxford Road, M13 9PT, Manchester, UK aut Dickson Alan Faculty of Life Sciences, The University of Manchester, The Michael Smith Building, Oxford Road, M13 9PT, Manchester, UK aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/8(2010-09-01), 657-663 1071-2690 46:8<657 2010 46 11626 https://doi.org/10.1007/s11626-010-9321-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9321-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Jiakai Faculty of Life Sciences, The University of Manchester, The Michael Smith Building, Oxford Road, M13 9PT, Manchester, UK aut NATIONALLICENCE 700 1- Dickson Alan Faculty of Life Sciences, The University of Manchester, The Michael Smith Building, Oxford Road, M13 9PT, Manchester, UK aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/8(2010-09-01), 657-663 1071-2690 46:8<657 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer