caa a22 4500 445292806 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100901xx s 000 0 eng 10.1007/s11626-010-9327-x doi (NATIONALLICENCE)springer-10.1007/s11626-010-9327-x Primary culture and characteristic morphologies of neurons from the cerebral ganglion of the mud crab, Scylla paramamosain [Elektronische Daten] [Yan Xu, Haihui Ye, Jun Ma, Huiyang Huang, Guizhong Wang] Crustacean neurons, obtained from the cerebral ganglion of the mud crab Scylla paramamosain, were successfully cultured in vitro. They maintained typical morphological characteristics and showed better outgrowth in modified Medium 199 (M199) medium than that in Liebowitz's L-15 medium. Fetal bovine serum (FBS), muscle extracts, and hemolymph of the mud crab S. paramamosain were added as supplements. Only 20% FBS could promote neuron outgrowth, while muscle extracts and hemolymph of S. paramamosain did not improve neuron outgrowth. For cell dissociation, both collagenase type I and trypsin worked well as determined by initial cell viability and following cell outgrowth potential. More than six kinds of cells with different morphological characteristics were identified in the neuron outgrowth. They were "small cells”, "veilers”, "branchers”, "multipolar cells”, "super-large cell”, and "bipolar cells”. Among all of the cells, bipolar cells were identified for the first time in crustacean neurons culture and they could live longer than other cells. The neurons could grow for more than a week before retraction and eventual degradation. The Society for In Vitro Biology, 2010 Cerebral ganglion nationallicence Neuron nationallicence Cell culture nationallicence Scylla paramamosain nationallicence Crustacean nationallicence Xu Yan Department of Oceanography, Xiamen University, 361005, Xiamen, China aut Ye Haihui Department of Oceanography, Xiamen University, 361005, Xiamen, China aut Ma Jun Department of Biology, University of Pennsylvania, 19104, Philadelphia, PA, USA aut Huang Huiyang Department of Oceanography, Xiamen University, 361005, Xiamen, China aut Wang Guizhong Department of Oceanography, Xiamen University, 361005, Xiamen, China aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/8(2010-09-01), 708-717 1071-2690 46:8<708 2010 46 11626 https://doi.org/10.1007/s11626-010-9327-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9327-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Yan Department of Oceanography, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 700 1- Ye Haihui Department of Oceanography, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 700 1- Ma Jun Department of Biology, University of Pennsylvania, 19104, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Huang Huiyang Department of Oceanography, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 700 1- Wang Guizhong Department of Oceanography, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/8(2010-09-01), 708-717 1071-2690 46:8<708 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer