caa a22 4500 445292814 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100901xx s 000 0 eng 10.1007/s11626-010-9330-2 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9330-2 Differential oxidative stress gene expression profile in mouse brain after proton exposure [Elektronische Daten] [Sudhakar Baluchamy, Ye Zhang, Prabakaran Ravichandran, Vani Ramesh, Ayodotun Sodipe, Joseph Hall, Olufisayo Jejelowo, Daila Gridley, Honglu Wu, Govindarajan Ramesh] Radiation is known to potentially interfere with cellular functions at all levels of cell organization. The radiation-induced stress response is very complex and involves altered expression of many genes. Identification of specific genes may allow the determination of pathways important in radiation responses. Although several radiation-related research have been studied extensively, the molecular and cellular processes affected by proton exposure remain poorly understood. Our earlier reports have shown that proton radiation induces reactive oxygen species (ROS) formation and lipid peroxidation and inhibits antioxidants, superoxide dismutase, and glutathione. Therefore, in this present study, we used quantitative real-time reverse transcription polymerase chain reaction approach and showed the modulation of several genes including oxidative stress, antioxidants defense mechanism, ROS metabolism, and oxygen transporters related genes expression in 2-Gy proton-exposed mouse brain. Literature evidences suggest that change in oxidants and antioxidants levels induce DNA damage, followed by cell death. In conclusion, changes in the gene profile of mouse brain after proton irradiation are complex and the exposed cells might undergo programmed cell death through alteration of genes responsible for oxidative stress signaling mechanism. The Society for In Vitro Biology, 2010 Proton nationallicence Real-time RT-PCR nationallicence Oxidative stress nationallicence Reactive oxygen species (ROS) nationallicence Baluchamy Sudhakar Molecular Toxicology Laboratory, Center for Biotechnology & Biomedical Sciences, Department of Biology, Norfolk State University, 23504, Norfolk, VA, USA aut Zhang Ye Radiation Biophysics Laboratory and Human Adaptation & Countermeasures Division, NASA Johnson Space Center, 77058, Houston, TX, USA aut Ravichandran Prabakaran Molecular Toxicology Laboratory, Center for Biotechnology & Biomedical Sciences, Department of Biology, Norfolk State University, 23504, Norfolk, VA, USA aut Ramesh Vani Molecular Toxicology Laboratory, Center for Biotechnology & Biomedical Sciences, Department of Biology, Norfolk State University, 23504, Norfolk, VA, USA aut Sodipe Ayodotun Department of Biology, Texas Southern University, 77004, Houston, TX, USA aut Hall Joseph Molecular Toxicology Laboratory, Center for Biotechnology & Biomedical Sciences, Department of Biology, Norfolk State University, 23504, Norfolk, VA, USA aut Jejelowo Olufisayo Department of Biology, Texas Southern University, 77004, Houston, TX, USA aut Gridley Daila Department of Radiation Medicine, Loma Linda University Medical Center, 92354, Loma Linda, CA, USA aut Wu Honglu Radiation Biophysics Laboratory and Human Adaptation & Countermeasures Division, NASA Johnson Space Center, 77058, Houston, TX, USA aut Ramesh Govindarajan Molecular Toxicology Laboratory, Center for Biotechnology & Biomedical Sciences, Department of Biology, Norfolk State University, 23504, Norfolk, VA, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/8(2010-09-01), 718-725 1071-2690 46:8<718 2010 46 11626 https://doi.org/10.1007/s11626-010-9330-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9330-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baluchamy Sudhakar Molecular Toxicology Laboratory, Center for Biotechnology & Biomedical Sciences, Department of Biology, Norfolk State University, 23504, Norfolk, VA, USA aut NATIONALLICENCE 700 1- Zhang Ye Radiation Biophysics Laboratory and Human Adaptation & Countermeasures Division, NASA Johnson Space Center, 77058, Houston, TX, USA aut NATIONALLICENCE 700 1- Ravichandran Prabakaran Molecular Toxicology Laboratory, Center for Biotechnology & Biomedical Sciences, Department of Biology, Norfolk State University, 23504, Norfolk, VA, USA aut NATIONALLICENCE 700 1- Ramesh Vani Molecular Toxicology Laboratory, Center for Biotechnology & Biomedical Sciences, Department of Biology, Norfolk State University, 23504, Norfolk, VA, USA aut NATIONALLICENCE 700 1- Sodipe Ayodotun Department of Biology, Texas Southern University, 77004, Houston, TX, USA aut NATIONALLICENCE 700 1- Hall Joseph Molecular Toxicology Laboratory, Center for Biotechnology & Biomedical Sciences, Department of Biology, Norfolk State University, 23504, Norfolk, VA, USA aut NATIONALLICENCE 700 1- Jejelowo Olufisayo Department of Biology, Texas Southern University, 77004, Houston, TX, USA aut NATIONALLICENCE 700 1- Gridley Daila Department of Radiation Medicine, Loma Linda University Medical Center, 92354, Loma Linda, CA, USA aut NATIONALLICENCE 700 1- Wu Honglu Radiation Biophysics Laboratory and Human Adaptation & Countermeasures Division, NASA Johnson Space Center, 77058, Houston, TX, USA aut NATIONALLICENCE 700 1- Ramesh Govindarajan Molecular Toxicology Laboratory, Center for Biotechnology & Biomedical Sciences, Department of Biology, Norfolk State University, 23504, Norfolk, VA, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/8(2010-09-01), 718-725 1071-2690 46:8<718 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer