caa a22 4500 445292822 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100901xx s 000 0 eng 10.1007/s11626-010-9326-y doi (NATIONALLICENCE)springer-10.1007/s11626-010-9326-y Development and characterization of a new epithelial cell line PSF from caudal fin of Green chromide, Etroplus suratensis (Bloch, 1790) [Elektronische Daten] [T. Swaminathan, Wazir Lakra, A. Gopalakrishnan, V. Basheer, B. Khushwaha, K. Sajeela] A new cell line [pearlspot fin (PSF)] has been developed from caudal fin of Etroplus suratensis, a brackish/freshwater fish cultivated in India. The cell line was maintained in Leibovitz's L-15 supplemented with 10% fetal bovine serum (FBS). The PSF cell line consisted predominantly of epithelial-like cells. The cells were able to grow at temperatures between 25°C and 32°C with optimum temperature of 28°C. The growth rate of PSF cells increased as the FBS proportion increased from 2% to 20% at 28°C with optimum growth at the concentration of 10% FBS. One marine fish virus (fish nodavirus) was tested on this cell line and found not susceptible. After confluency, the cells were subcultured with a split ratio of 1:2. The cells showed epithelial-like morphology and reached confluency on the third d after subculture. Polymerase chain reaction amplification of mitochondrial 16S rRNA and COI indicated identity of this cell line with those reported from this fish species, confirming that the cell line was of pearlspot origin. The cells were successfully cryopreserved and revived at the tenth, 25th, and 35th passages. The bacterial extracellular products from Vibrio cholerae MTCC 3904 were found to be toxic to PSF. Karyotyping analysis indicated that the modal chromosome number was 48. The Society for In Vitro Biology, 2010 Green chromide nationallicence Pearlspot nationallicence Etroplus suratensis nationallicence PSF cell line nationallicence Swaminathan T. National Bureau of Fish Genetic Resources Cochin Unit, CMFRI Campus, P.O. Number 1603, 682018, Kochi, India aut Lakra Wazir National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, 226002, Lucknow, India aut Gopalakrishnan A. National Bureau of Fish Genetic Resources Cochin Unit, CMFRI Campus, P.O. Number 1603, 682018, Kochi, India aut Basheer V. National Bureau of Fish Genetic Resources Cochin Unit, CMFRI Campus, P.O. Number 1603, 682018, Kochi, India aut Khushwaha B. National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, 226002, Lucknow, India aut Sajeela K. National Bureau of Fish Genetic Resources Cochin Unit, CMFRI Campus, P.O. Number 1603, 682018, Kochi, India aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/8(2010-09-01), 647-656 1071-2690 46:8<647 2010 46 11626 https://doi.org/10.1007/s11626-010-9326-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9326-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Swaminathan T. National Bureau of Fish Genetic Resources Cochin Unit, CMFRI Campus, P.O. Number 1603, 682018, Kochi, India aut NATIONALLICENCE 700 1- Lakra Wazir National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, 226002, Lucknow, India aut NATIONALLICENCE 700 1- Gopalakrishnan A. National Bureau of Fish Genetic Resources Cochin Unit, CMFRI Campus, P.O. Number 1603, 682018, Kochi, India aut NATIONALLICENCE 700 1- Basheer V. National Bureau of Fish Genetic Resources Cochin Unit, CMFRI Campus, P.O. Number 1603, 682018, Kochi, India aut NATIONALLICENCE 700 1- Khushwaha B. National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, 226002, Lucknow, India aut NATIONALLICENCE 700 1- Sajeela K. National Bureau of Fish Genetic Resources Cochin Unit, CMFRI Campus, P.O. Number 1603, 682018, Kochi, India aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/8(2010-09-01), 647-656 1071-2690 46:8<647 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer