caa a22 4500 445292849 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100901xx s 000 0 eng 10.1007/s11626-010-9324-0 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9324-0 Coexpression of glutamate vesicular transporter (VGLUT1) and choline acetyltransferase (ChAT) proteins in fetal rat hippocampal neurons in culture [Elektronische Daten] [Neelima Bhargava, Mainak Das, Darin Edwards, Maria Stancescu, Jung-Fong Kang, James Hickman] A very small population of choline acetyltransferase (ChAT) immunoreactive cells is observed in all layers of the adult hippocampus. This is the intrinsic source of the hippocampal cholinergic innervation, in addition to the well-established septo-hippocampal cholinergic projection. This study aimed at quantifying and identifying the origin of this small population of ChAT-immunoreactive cells in the hippocampus at early developmental stages, by culturing the fetal hippocampal neurons in serum-free culture and on a patternable, synthetic silane substrate N-1 [3-(trimethoxysilyl) propyl] diethylenetriamine. Using this method, a large proportion of glutamatergic (glutamate vesicular transporter, VGLUT1-immunoreactive) neurons, a small fraction of GABAergic (GABA-immunoreactive) neurons, and a large proportion of cholinergic (ChAT-immunoreactive) neurons were observed in the culture. Interestingly, most of the glutamatergic neurons that expressed glutamate vesicular transporter (VGLUT1) also co-expressed ChAT proteins. On the contrary, when the cultures were double-stained with GABA and ChAT, colocalization was not observed. Neonatal and adult rat hippocampal neurons were also cultured to verify whether these more mature neurons also co-express VGLUT1 and ChAT proteins in culture. Colocalization of VGLUT1 and ChAT in these relatively more mature neurons was not observed. One possible explanation for this observation is that the neurons have the ability to synthesize multiple neurotransmitters at a very early stage of development and then with time follows a complex, combinatorial strategy of electrochemical coding to determine their final fate. The Society for In Vitro Biology, 2010 Adult hippocampal neuron nationallicence Fetal nationallicence VGLUT1 nationallicence ChAT nationallicence Serum-free culture nationallicence Bhargava Neelima NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Suite 400, 32826, Orlando, FL, USA aut Das Mainak NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Suite 400, 32826, Orlando, FL, USA aut Edwards Darin NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Suite 400, 32826, Orlando, FL, USA aut Stancescu Maria NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Suite 400, 32826, Orlando, FL, USA aut Kang Jung-Fong NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Suite 400, 32826, Orlando, FL, USA aut Hickman James NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Suite 400, 32826, Orlando, FL, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/8(2010-09-01), 685-692 1071-2690 46:8<685 2010 46 11626 https://doi.org/10.1007/s11626-010-9324-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9324-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bhargava Neelima NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Suite 400, 32826, Orlando, FL, USA aut NATIONALLICENCE 700 1- Das Mainak NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Suite 400, 32826, Orlando, FL, USA aut NATIONALLICENCE 700 1- Edwards Darin NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Suite 400, 32826, Orlando, FL, USA aut NATIONALLICENCE 700 1- Stancescu Maria NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Suite 400, 32826, Orlando, FL, USA aut NATIONALLICENCE 700 1- Kang Jung-Fong NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Suite 400, 32826, Orlando, FL, USA aut NATIONALLICENCE 700 1- Hickman James NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Suite 400, 32826, Orlando, FL, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/8(2010-09-01), 685-692 1071-2690 46:8<685 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer