caa a22 4500 445292857 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100501xx s 000 0 eng 10.1007/s11626-009-9259-5 doi (NATIONALLICENCE)springer-10.1007/s11626-009-9259-5 Hepatopancreas cell cultures from mud crab, Scylla paramamosain [Elektronische Daten] [Hui Zeng, Haihui Ye, Shaojing Li, Guizhong Wang, Jingru Huang] Hepatopancreas is an important digestive and endocrine organ in crustacean. However, there are few reports on cell cultures from crabs. Here, the cell cultures of hepatopancreas from Scylla paramamosain was studied in vitro. Both the primary cell culture and subculture were grown in Leibovitz' L-15 medium, M199 medium, or a specially designed medium for S. paramamosain (MSP). The results showed that hepatopancreas cells in vitro grew in compact clusters in 2-3d. Four types of cells could be identified. They were embryo cells, fibrillar cells, resorptive cells, and blister-like cells, respectively. Some of these cells could be subcultured for three generations. The MSP supported the best survival of these hepatopancreas cells, while M199 medium was the least effective of these three media. Fetal bovine serum and crab muscle extracts as supplements stimulated growth, but the crab hemolymph inhibited cell growth. Taken together, MSP is an appropriate medium for hepatopancreas cell cultures from S. paramamosain and can support cultures through several passages. The Society for In Vitro Biology, 2009 Hepatopancreas nationallicence Cell culture nationallicence Subculture nationallicence Scylla paramamosain nationallicence Zeng Hui Department of Oceanography, Xiamen University, 361005, Xiamen, China aut Ye Haihui Department of Oceanography, Xiamen University, 361005, Xiamen, China aut Li Shaojing Department of Oceanography, Xiamen University, 361005, Xiamen, China aut Wang Guizhong Department of Oceanography, Xiamen University, 361005, Xiamen, China aut Huang Jingru Department of Oceanography, Xiamen University, 361005, Xiamen, China aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 431-437 1071-2690 46:5<431 2010 46 11626 https://doi.org/10.1007/s11626-009-9259-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9259-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zeng Hui Department of Oceanography, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 700 1- Ye Haihui Department of Oceanography, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 700 1- Li Shaojing Department of Oceanography, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 700 1- Wang Guizhong Department of Oceanography, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 700 1- Huang Jingru Department of Oceanography, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 431-437 1071-2690 46:5<431 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer