caa a22 4500 445292865 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100501xx s 000 0 eng 10.1007/s11626-009-9267-5 doi (NATIONALLICENCE)springer-10.1007/s11626-009-9267-5 Cardiac microvascular endothelial cells express and release nerve growth factor but not fibroblast growth factor-2 [Elektronische Daten] [Shimon Lecht, Carola Foerster, Hadar Arien-Zakay, Cezary Marcinkiewicz, Philip Lazarovici, Peter Lelkes] Endothelial cells (ECs) from different vascular beds not only display common characteristics but are also quite heterogeneous in terms of expression and secretion of neuro-angiogenic factors, which may help explain some of their distinct physiological roles. We investigated by RT-PCR the gene expression, by PC12 bioassay the neurotropic activity, and by ELISAs the levels of NGF and FGF-2 using conditioned medium collected from cultures of ECs derived from myocardial and cerebral capillaries. While NGF was expressed and released by both cell types, FGF-2 was expressed and released solely by the brain but not heart ECs. Oxygen-glucose deprivation (ischemic) insult blocked NGF secretion from heart and brain ECs and inhibited by 70% the secretion of FGF-2 from brain ECs. We propose that the differential expression of NGF and FGF-2 in heart and brain EC cultures reflect heterogeneity on demand of the microcapillary components and the surrounding microenvironment for a proper tissue-specific homeostasis. The Society for In Vitro Biology, 2010 Brain nationallicence Endothelial heterogeneity nationallicence FGF-2 nationallicence Heart nationallicence NGF nationallicence Lecht Shimon The School of Pharmacy Institute for Drug Research, Faculty of Medicine, The Hebrew University of Jerusalem, 91120, Jerusalem, Israel aut Foerster Carola Institute of Anatomy and Cell Biology, University of Wurzburg, Wurzburg, Germany aut Arien-Zakay Hadar The School of Pharmacy Institute for Drug Research, Faculty of Medicine, The Hebrew University of Jerusalem, 91120, Jerusalem, Israel aut Marcinkiewicz Cezary Department of Biology, Temple University, Philadelphia, PA, USA aut Lazarovici Philip The School of Pharmacy Institute for Drug Research, Faculty of Medicine, The Hebrew University of Jerusalem, 91120, Jerusalem, Israel aut Lelkes Peter Laboratory of Cellular Tissue Engineering, School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 469-476 1071-2690 46:5<469 2010 46 11626 https://doi.org/10.1007/s11626-009-9267-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9267-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lecht Shimon The School of Pharmacy Institute for Drug Research, Faculty of Medicine, The Hebrew University of Jerusalem, 91120, Jerusalem, Israel aut NATIONALLICENCE 700 1- Foerster Carola Institute of Anatomy and Cell Biology, University of Wurzburg, Wurzburg, Germany aut NATIONALLICENCE 700 1- Arien-Zakay Hadar The School of Pharmacy Institute for Drug Research, Faculty of Medicine, The Hebrew University of Jerusalem, 91120, Jerusalem, Israel aut NATIONALLICENCE 700 1- Marcinkiewicz Cezary Department of Biology, Temple University, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Lazarovici Philip The School of Pharmacy Institute for Drug Research, Faculty of Medicine, The Hebrew University of Jerusalem, 91120, Jerusalem, Israel aut NATIONALLICENCE 700 1- Lelkes Peter Laboratory of Cellular Tissue Engineering, School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 469-476 1071-2690 46:5<469 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer