caa a22 4500 445292881 CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100501xx s 000 0 eng 10.1007/s11626-009-9268-4 doi (NATIONALLICENCE)springer-10.1007/s11626-009-9268-4 Electrical stimulation of cultured lepidopteran dorsal vessel tissue: an experiment for development of bioactuators [Elektronische Daten] [Yoshitake Akiyama, Kikuo Iwabuchi, Yuji Furukawa, Keisuke Morishima] An insect dorsal vessel (DV) is well suited for a bioactuator since it is capable of contracting autonomously, and its tissue and cells are more environmentally robust under culturing conditions compared with mammalian tissue. In this study, electrical pulse stimulation was examined so as to regulate a bioactuator using the DV tissue. The DV tissue of a larva of Ctenoplusia agnate was assembled on a micropillar array, which was stimulated after culturing for about 3wk. The contraction of the DV tissue was evaluated by image analysis to measure lateral displacements at the micropillar top. As a result, suitable stimulation conditions in a 35-mm petri dish were determined as: applied voltage of 10V with 20-ms duration. Next, the time lag between the onset of electrical stimulus and the onset of mechanical contraction (electromechanical delay (EMD)) was estimated. A light-emitting diode (LED) was connected serially with the petri dish, and the LED flashed when electrical pulses were given. Movie images were analyzed in which electrical pulses made the DV tissue contract and the LED flashed virtually simultaneously; from these, the EMD was estimated as approximately 50ms. These results suggest that the electrical pulse stimulation is capable of regulating the DV tissue, and the micropillar array is a useful biological tool to investigate physiological properties of muscle tissue. The Society for In Vitro Biology, 2009 Bioactuator nationallicence Electrical stimulation nationallicence Dorsal vessel nationallicence Electromechanical delay nationallicence Insect tissue culture nationallicence Akiyama Yoshitake Department of Bio-Mechanics and Intelligent Systems, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, 184-8588, Koganei, Tokyo, Japan aut Iwabuchi Kikuo Department of Applied Molecular Biology and Biochemistry, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, 183-8509, Fuchu, Tokyo, Japan aut Furukawa Yuji Department of Mechanical Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, 184-8588, Koganei, Tokyo, Japan aut Morishima Keisuke Department of Bio-Mechanics and Intelligent Systems, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, 184-8588, Koganei, Tokyo, Japan aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 411-415 1071-2690 46:5<411 2010 46 11626 https://doi.org/10.1007/s11626-009-9268-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9268-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Akiyama Yoshitake Department of Bio-Mechanics and Intelligent Systems, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, 184-8588, Koganei, Tokyo, Japan aut NATIONALLICENCE 700 1- Iwabuchi Kikuo Department of Applied Molecular Biology and Biochemistry, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, 183-8509, Fuchu, Tokyo, Japan aut NATIONALLICENCE 700 1- Furukawa Yuji Department of Mechanical Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, 184-8588, Koganei, Tokyo, Japan aut NATIONALLICENCE 700 1- Morishima Keisuke Department of Bio-Mechanics and Intelligent Systems, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, 184-8588, Koganei, Tokyo, Japan aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 411-415 1071-2690 46:5<411 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer