caa a22 4500 44529289X CHVBK 20180317142533.0 cr unu---uuuuu 170323e20100501xx s 000 0 eng 10.1007/s11626-009-9265-7 doi (NATIONALLICENCE)springer-10.1007/s11626-009-9265-7 Newly established cell lines from mouse oral epithelium regenerate teeth when combined with dental mesenchyme [Elektronische Daten] [Chiho Takahashi, Hiroyuki Yoshida, Akihiko Komine, Kazuhisa Nakao, Takashi Tsuji, Yasuhiro Tomooka] The present study attempted to examine whether clonal cell lines of the oral epithelium can differentiate into ameloblasts and regenerate tooth when combined with dental germ mesenchyme. Clonal cell lines with a distinct morphology were established from the oral epithelium of p53-deficient fetal mice at embryonic day18 (E18). The strain of mouse is shown to be a useful source for establishing clonal and immortalized cell lines from various tissues and at various stages of development. Tooth morphogenesis is almost completed and the oral epithelium is segregated from the dental epithelium at E18. In RT-PCR analysis of cell lines, mucosal epithelial markers (cytokeratin 14) were detected, but ameloblast markers such as amelogenin and ameloblastin were not detected when cells were cultured on plastic dish. They formed stratified epithelia and expressed a specific differentiation marker (CK13) in the upper layer when cultured on feeder layer or on collagen gel for 1-3wk, demonstrating that they are of oral mucosa origin. Next, bioengineered tooth germs were prepared with cell lines and fetal molar mesenchymal tissues and implanted under kidney capsule for 2-3wk. Five among six cell lines regenerated calcified structures as seen in natural tooth. Our results indicate that some oral epithelial cells at E18 possess the capability to differentiate into ameloblasts. Furthermore, cell lines established in the present study are useful models to study processes in tooth organogenesis and tooth regeneration. The Author(s), 2009 Oral epithelium nationallicence Immortalized cell lines nationallicence Tooth regeneration nationallicence p53 -deficient mouse nationallicence Takahashi Chiho Department of Biological Science and Technology and Tissue Engineering Research Center, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut Yoshida Hiroyuki Department of Biological Science and Technology and Tissue Engineering Research Center, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut Komine Akihiko Department of Biological Science and Technology and Tissue Engineering Research Center, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut Nakao Kazuhisa Department of Biological Science and Technology and Tissue Engineering Research Center, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut Tsuji Takashi Department of Biological Science and Technology and Tissue Engineering Research Center, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut Tomooka Yasuhiro Department of Biological Science and Technology and Tissue Engineering Research Center, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 457-468 1071-2690 46:5<457 2010 46 11626 https://doi.org/10.1007/s11626-009-9265-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9265-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takahashi Chiho Department of Biological Science and Technology and Tissue Engineering Research Center, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Yoshida Hiroyuki Department of Biological Science and Technology and Tissue Engineering Research Center, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Komine Akihiko Department of Biological Science and Technology and Tissue Engineering Research Center, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Nakao Kazuhisa Department of Biological Science and Technology and Tissue Engineering Research Center, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Tsuji Takashi Department of Biological Science and Technology and Tissue Engineering Research Center, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Tomooka Yasuhiro Department of Biological Science and Technology and Tissue Engineering Research Center, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 457-468 1071-2690 46:5<457 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer