caa a22 4500 445292903 CHVBK 20180317142534.0 cr unu---uuuuu 170323e20100501xx s 000 0 eng 10.1007/s11626-009-9260-z doi (NATIONALLICENCE)springer-10.1007/s11626-009-9260-z Development of a scaffoldless three-dimensional engineered nerve using a nerve-fibroblast co-culture [Elektronische Daten] [Jennifer Baltich, Leah Hatch-Vallier, Aaron Adams, Ellen Arruda, Lisa Larkin] Nerve grafts are often required to replace tissue damaged by disease, surgery, or extensive trauma. Limitations such as graft availability, donor site morbidity, and immune rejection have led investigators to develop strategies to engineer nerve tissue. The goal of this study was to fabricate a scaffoldless three-dimensional (3D) nerve construct using a co-culture of fetal nerve cells with a fibroblast monolayer and allow the co-culture to remodel into a 3D construct with an external fibroblast layer and an internal core of interconnected neuronal cells. Primary fibroblasts were seeded on laminin-coated plates and allowed to form a confluent monolayer. Neural cells isolated from E-15 spinal cords were seeded on top of the fibroblast monolayer and allowed to form a networked monolayer across the monolayer of fibroblasts. Media shifts initiated contraction of the fibroblast monolayer and a remodeling of the co-culture into a 3D construct held statically in place by the two constraint pins. Immunohistochemistry using S100 (Schwann cell), β3-tubulin, DAPI, and collagen I indicated an inner core of nerve cells surrounded by an external layer of fibroblasts. Conduction velocities of the 3D nerve and control (fibroblast-only) constructs were measured in vitro and compared to in vivo measures of neonatal sciatic nerve. The conduction velocities of the nerve constructs were comparable to 24-d-old neonatal nerve. The presence of Schwann cells and the ability to conduct neuronal signals in vitro suggest the scaffoldless 3D nerve constructs will be a viable option for nerve repair. The Society for In Vitro Biology, 2009 Nerve nationallicence Tissue engineering nationallicence Fibroblast nationallicence Baltich Jennifer Biomedical Engineering, University of Michigan, Room #2025 Biomedical Science Research Building (BSRB), 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut Hatch-Vallier Leah Biomedical Engineering, University of Michigan, Room #2025 Biomedical Science Research Building (BSRB), 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut Adams Aaron Molecular and Integrated Physiology, University of Michigan, Room #2025 Biomedical Science Research Building (BSRB), 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut Arruda Ellen Mechanical Engineering, University of Michigan, 3126 GGB, 48109-2125, Ann Arbor, MI, USA aut Larkin Lisa Biomedical Engineering, University of Michigan, Room #2025 Biomedical Science Research Building (BSRB), 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 438-444 1071-2690 46:5<438 2010 46 11626 https://doi.org/10.1007/s11626-009-9260-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9260-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baltich Jennifer Biomedical Engineering, University of Michigan, Room #2025 Biomedical Science Research Building (BSRB), 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Hatch-Vallier Leah Biomedical Engineering, University of Michigan, Room #2025 Biomedical Science Research Building (BSRB), 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Adams Aaron Molecular and Integrated Physiology, University of Michigan, Room #2025 Biomedical Science Research Building (BSRB), 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Arruda Ellen Mechanical Engineering, University of Michigan, 3126 GGB, 48109-2125, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Larkin Lisa Biomedical Engineering, University of Michigan, Room #2025 Biomedical Science Research Building (BSRB), 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 438-444 1071-2690 46:5<438 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer