caa a22 4500 44529292X CHVBK 20180317142534.0 cr unu---uuuuu 170323e20100501xx s 000 0 eng 10.1007/s11626-009-9261-y doi (NATIONALLICENCE)springer-10.1007/s11626-009-9261-y Dexamethasone protects cultured rat hepatocytes against cadmium toxicity: involvement of cellular thiols [Elektronische Daten] [Andrea Ferrigno, Cesarina Gregotti, Plinio Richelmi, Mariapia Vairetti] In the present study, the effects of dexamethasone on cadmium-induced toxicity were evaluated in isolated rat hepatocytes. Hepatocytes were cultured for 24h in William's E medium containing fetal calf serum (10%), insulin (0.1IU/ml), and glucagon (0.01μM) in the absence or presence of 0.1μM dexamethasone. Cadmium chloride, 5 or 10μM, was added to the medium and the toxicity was evaluated for up to 48h after treatment. Lactate dehydrogenase (LDH) release, the reduced and oxidized glutathione ratio (GSH/GSSG), protein-SH groups, and lipid peroxidation levels were evaluated. Cadmium induced a dose- and time-dependent LDH release in control hepatocytes at 24h (Cd 10μM 42%) while hepatocytes pretreated with dexamethasone showed lower necrosis (Cd 10μM 12% at 24h). GSH/GSSH ratio and protein-SH groups were higher while lipid peroxidation was lower in dexamethasone-treated hepatocytes as compared with untreated cells. In conclusion, cadmium toxicity was associated with an increase in intracellular oxidative stress responsible for accelerated cell death. The use of dexamethasone prevented cadmium damage, suggesting that the cytoprotective action of this hormone is related to its effect in preventing changes in thiols such as glutathione and protein-SH groups. The Society for In Vitro Biology, 2009 Liver nationallicence Cadmium nationallicence Dexamethasone nationallicence Glutathione nationallicence SH groups nationallicence Ferrigno Andrea Department of Internal Medicine and Therapeutics, University of Pavia, Via Ferrata 9A, 27100, Pavia, Italy aut Gregotti Cesarina Department of Internal Medicine and Therapeutics, University of Pavia, Via Ferrata 9A, 27100, Pavia, Italy aut Richelmi Plinio Department of Internal Medicine and Therapeutics, University of Pavia, Via Ferrata 9A, 27100, Pavia, Italy aut Vairetti Mariapia Department of Internal Medicine and Therapeutics, University of Pavia, Via Ferrata 9A, 27100, Pavia, Italy aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 445-449 1071-2690 46:5<445 2010 46 11626 https://doi.org/10.1007/s11626-009-9261-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9261-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ferrigno Andrea Department of Internal Medicine and Therapeutics, University of Pavia, Via Ferrata 9A, 27100, Pavia, Italy aut NATIONALLICENCE 700 1- Gregotti Cesarina Department of Internal Medicine and Therapeutics, University of Pavia, Via Ferrata 9A, 27100, Pavia, Italy aut NATIONALLICENCE 700 1- Richelmi Plinio Department of Internal Medicine and Therapeutics, University of Pavia, Via Ferrata 9A, 27100, Pavia, Italy aut NATIONALLICENCE 700 1- Vairetti Mariapia Department of Internal Medicine and Therapeutics, University of Pavia, Via Ferrata 9A, 27100, Pavia, Italy aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 445-449 1071-2690 46:5<445 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer