caa a22 4500 445292938 CHVBK 20180317142534.0 cr unu---uuuuu 170323e20100501xx s 000 0 eng 10.1007/s11626-009-9262-x doi (NATIONALLICENCE)springer-10.1007/s11626-009-9262-x Cultures of human tracheal gland cells of mucous or serous phenotype [Elektronische Daten] [Walter Finkbeiner, Lorna Zlock, Irum Mehdi, Jonathan Widdicombe] There are two main epithelial cell types in the secretory tubules of mammalian glands: serous and mucous. The former is believed to secrete predominantly water and antimicrobials, the latter mucins. Primary cultures of human airway gland epithelium have been available for almost 20yr, but they are poorly differentiated and lack clear features of either serous or mucous cells. In this study, by varying growth supports and media, we have produced cultures from human airway glands that in terms of their ultrastructure and secretory products resemble either mucous or serous cells. Of four types of porous-bottomed insert tested, polycarbonate filters (Transwells) most strongly promoted the mucous phenotype. Coupled with the addition of epidermal growth factor (EGF), this growth support produced "mucous” cells that contained the large electron-lucent granules characteristic of native mucous cells, but lacked the small electron-dense granules characteristic of serous cells. Furthermore, they showed high levels of mucin secretion and low levels of release of lactoferrin and lysozyme (markers of native serous cells). By contrast, growth on polyethylene terephthalate filters (Cyclopore) in medium lacking EGF produced "serous” cells in which small electron-dense granules replaced the electron-lucent ones, and the cells had high levels of lactoferrin and lysozyme but low levels of mucins. Measurements of transepithelial resistance and short-circuit current showed that both "serous” and "mucous” cell cultures possessed tight junctions, had become polarized, and were actively secreting Cl. The Author(s), 2009 Airway mucous gland nationallicence Serous cell nationallicence Mucous cell nationallicence Chloride secretion nationallicence Finkbeiner Walter Department of Pathology, University of California, San Francisco, CA, USA aut Zlock Lorna Department of Pathology, University of California, San Francisco, CA, USA aut Mehdi Irum Department of Pathology, University of California, San Francisco, CA, USA aut Widdicombe Jonathan Department of Physiology & Membrane Biology, University of California-Davis, 95616-8664, Davis, CA, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 450-456 1071-2690 46:5<450 2010 46 11626 https://doi.org/10.1007/s11626-009-9262-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9262-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Finkbeiner Walter Department of Pathology, University of California, San Francisco, CA, USA aut NATIONALLICENCE 700 1- Zlock Lorna Department of Pathology, University of California, San Francisco, CA, USA aut NATIONALLICENCE 700 1- Mehdi Irum Department of Pathology, University of California, San Francisco, CA, USA aut NATIONALLICENCE 700 1- Widdicombe Jonathan Department of Physiology & Membrane Biology, University of California-Davis, 95616-8664, Davis, CA, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 450-456 1071-2690 46:5<450 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer